Single-cell eQTL mapping of human endogenous retroviruses reveals cell type-specific genetic regulation in autoimmune diseases

Abstract Human endogenous retroviruses constitute a significant portion of the human genome and play complex roles in gene regulation and disease processes. However, the expression pattern and disease associations of specific retroviral loci remain pooly understood. This study examines the expressio...

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Main Authors: Fan Zhu, Yi Liu, Jiehao Lei, Xinxing Li, Zexu Jiang, Xuan Dong, Ying Gu, Young Li
Format: Article
Language:English
Published: Nature Portfolio 2025-08-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-025-62779-7
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author Fan Zhu
Yi Liu
Jiehao Lei
Xinxing Li
Zexu Jiang
Xuan Dong
Ying Gu
Young Li
author_facet Fan Zhu
Yi Liu
Jiehao Lei
Xinxing Li
Zexu Jiang
Xuan Dong
Ying Gu
Young Li
author_sort Fan Zhu
collection DOAJ
description Abstract Human endogenous retroviruses constitute a significant portion of the human genome and play complex roles in gene regulation and disease processes. However, the expression pattern and disease associations of specific retroviral loci remain pooly understood. This study examines the expression and regulatory mechanisms of these retroviral elements in immune cells. Utilizing single-cell RNA sequencing data from peripheral blood mononuclear cells, we identify 41,460 expressed retroviral loci, with 1936 showing cell type-specific expression. We further detect 3463 conditionally independent expression quantitative trait loci linked to retroviral elements, highlighting their potential role in mediating genetic variants and disease associations. Notably, these retroviral sequences associate significantly with autoimmune diseases, with specific loci demonstrating pleiotropic associations with disease-related genes. Our findings suggest that these elements are not merely genomic remnants but active participants in cellular regulation and disease progression. This work advances understanding of human-retroviral coevolution and highlights potential therapeutic targets in immune disorders.
format Article
id doaj-art-20c86eb53b4347289aa23f87babb2437
institution Kabale University
issn 2041-1723
language English
publishDate 2025-08-01
publisher Nature Portfolio
record_format Article
series Nature Communications
spelling doaj-art-20c86eb53b4347289aa23f87babb24372025-08-20T03:43:16ZengNature PortfolioNature Communications2041-17232025-08-0116111410.1038/s41467-025-62779-7Single-cell eQTL mapping of human endogenous retroviruses reveals cell type-specific genetic regulation in autoimmune diseasesFan Zhu0Yi Liu1Jiehao Lei2Xinxing Li3Zexu Jiang4Xuan Dong5Ying Gu6Young Li7BGI ResearchBGI ResearchBGI ResearchBGI ResearchBGI ResearchBGI ResearchBGI ResearchBGI ResearchAbstract Human endogenous retroviruses constitute a significant portion of the human genome and play complex roles in gene regulation and disease processes. However, the expression pattern and disease associations of specific retroviral loci remain pooly understood. This study examines the expression and regulatory mechanisms of these retroviral elements in immune cells. Utilizing single-cell RNA sequencing data from peripheral blood mononuclear cells, we identify 41,460 expressed retroviral loci, with 1936 showing cell type-specific expression. We further detect 3463 conditionally independent expression quantitative trait loci linked to retroviral elements, highlighting their potential role in mediating genetic variants and disease associations. Notably, these retroviral sequences associate significantly with autoimmune diseases, with specific loci demonstrating pleiotropic associations with disease-related genes. Our findings suggest that these elements are not merely genomic remnants but active participants in cellular regulation and disease progression. This work advances understanding of human-retroviral coevolution and highlights potential therapeutic targets in immune disorders.https://doi.org/10.1038/s41467-025-62779-7
spellingShingle Fan Zhu
Yi Liu
Jiehao Lei
Xinxing Li
Zexu Jiang
Xuan Dong
Ying Gu
Young Li
Single-cell eQTL mapping of human endogenous retroviruses reveals cell type-specific genetic regulation in autoimmune diseases
Nature Communications
title Single-cell eQTL mapping of human endogenous retroviruses reveals cell type-specific genetic regulation in autoimmune diseases
title_full Single-cell eQTL mapping of human endogenous retroviruses reveals cell type-specific genetic regulation in autoimmune diseases
title_fullStr Single-cell eQTL mapping of human endogenous retroviruses reveals cell type-specific genetic regulation in autoimmune diseases
title_full_unstemmed Single-cell eQTL mapping of human endogenous retroviruses reveals cell type-specific genetic regulation in autoimmune diseases
title_short Single-cell eQTL mapping of human endogenous retroviruses reveals cell type-specific genetic regulation in autoimmune diseases
title_sort single cell eqtl mapping of human endogenous retroviruses reveals cell type specific genetic regulation in autoimmune diseases
url https://doi.org/10.1038/s41467-025-62779-7
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