Polycationic Lipids Translocate Lipopolysaccharide into HeLa Cells

We have investigated the ability of LipofectAMINE™, a polycationic lipid reagent used in DNA transfection, to translocate E. coli lipopolysaccharide (LPS) into HeLa cells. Although HeLa cells did not spontaneously take up fluorescein isothiocyanatelabelled LPS (FITC-LPS) from the culture medium, the...

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Bibliographic Details
Main Authors: Jodene R. Eldstrom, Kimberly La, David A. Mathers
Format: Article
Language:English
Published: Taylor & Francis Group 2000-03-01
Series:BioTechniques
Online Access:https://www.future-science.com/doi/10.2144/00283st10
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