Novel Vector for Generating RNAs with Defined 3′ Ends and Its Use in Antiviral Strategies
A novel transcription system was constructed that allows trimming of 3′ termini of RNA transcripts in E. coli by endogenous RNase P. Here, the sequence of tRNASer from E. coli fused downstream of the target sequence directs posttranscriptional cleavage 3′ of the target sequence. As a first-target MN...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
1998-01-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/98241st05 |
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