CRISPR/FnCas12a-mediated efficient multiplex and iterative genome editing in bacterial plant pathogens without donor DNA templates.

CRISPR/FnCas12a-mediated efficient multiplex and iterative genome editing in bacterial plant pathogens without donor DNA templates.

CRISPR-based genome editing technology is revolutionizing prokaryotic research, but it has been rarely studied in bacterial plant pathogens. Here, we have developed a targeted genome editing method with no requirement of donor templates for convenient and efficient gene knockout in Xanthomonas oryza...

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Bibliographic Details
Main Authors: Fang Yan, Jingwen Wang, Sujie Zhang, Zhenwan Lu, Shaofang Li, Zhiyuan Ji, Congfeng Song, Gongyou Chen, Jin Xu, Jie Feng, Xueping Zhou, Huanbin Zhou
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2023-01-01
Series:PLoS Pathogens
Online Access:https://journals.plos.org/plospathogens/article/file?id=10.1371/journal.ppat.1010961&type=printable
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https://journals.plos.org/plospathogens/article/file?id=10.1371/journal.ppat.1010961&type=printable

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