Simultaneous detection and differentiation of Mycobacterium tuberculosis and nontuberculous mycobacteria in smear-negative sputum by a multiplex PCR assay: a clinical feasibility study

Simultaneous detection and differentiation of Mycobacterium tuberculosis and nontuberculous mycobacteria in smear-negative sputum by a multiplex PCR assay: a clinical feasibility study

ABSTRACT Accurate and rapid differentiation of Mycobacterium tuberculosis (M. tuberculosis) and nontuberculous mycobacteria (NTM) remains challenging, especially in smear-negative samples. Routine diagnostic methods often lack specificity and rapidity, highlighting the urgency of developing applicat...

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Main Authors: Long Xie, Jing Cao, Yong Yang, Shao-Long Jiang, Jin-Lin Tan, Jia Zhang, Ze-Fan Ruan, Li Chen, Da-Yong Xu, Zhong Chen, Ming-Xiang Huang, Xi-Wen Jiang
Format: Article
Language:English
Published: American Society for Microbiology 2025-07-01
Series:Microbiology Spectrum
Subjects:
Mycobacterium tuberculosis
nontuberculous mycobacteria
multiplex TaqMan PCR
assay development and clinical feasibility evaluation
sputum detection
Online Access:https://journals.asm.org/doi/10.1128/spectrum.02316-24
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https://journals.asm.org/doi/10.1128/spectrum.02316-24

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