Mechanistic investigation of a soybean protein isolate–soyasaponin–pectin composite system on soyasaponin bitterness in an acidic environment

Herein, a soybean protein isolate–soyasaponin (SPI–Ssa) composite system was constructed to mimic a complex, multifaceted soybean product system. This system was stabilized via the addition of high-methoxy pectin (HMP) and low-methoxy pectin (LMP), and the bitter taste of Ssa was modulated. HMP and...

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Main Authors: Lijie Zhu, Yingyan Li, He Li, Dayun Zhao, Xinqi Liu, Qian Shen, Qi Zhang, Qingyun Lv, Xiuying Liu, Wenping Ding
Format: Article
Language:English
Published: Elsevier 2025-04-01
Series:Food Chemistry: X
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Online Access:http://www.sciencedirect.com/science/article/pii/S2590157525003475
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author Lijie Zhu
Yingyan Li
He Li
Dayun Zhao
Xinqi Liu
Qian Shen
Qi Zhang
Qingyun Lv
Xiuying Liu
Wenping Ding
author_facet Lijie Zhu
Yingyan Li
He Li
Dayun Zhao
Xinqi Liu
Qian Shen
Qi Zhang
Qingyun Lv
Xiuying Liu
Wenping Ding
author_sort Lijie Zhu
collection DOAJ
description Herein, a soybean protein isolate–soyasaponin (SPI–Ssa) composite system was constructed to mimic a complex, multifaceted soybean product system. This system was stabilized via the addition of high-methoxy pectin (HMP) and low-methoxy pectin (LMP), and the bitter taste of Ssa was modulated. HMP and LMP were compared using sensory evaluation and an electronic tongue assay, revealing that HMP was more effective in suppressing bitterness. Moreover, the interfacial properties and structural characteristics of the SPI–Ssa–HMP composite system were determined at different pH values, and the effect of composite coalescence on the adsorption of Ssa in acidic and neutral environments was investigated. The network structure formed between SPI and HMP was determined by differential scanning isothermal titration calorimetry and Fourier transform infrared spectroscopy. The formation of the network structure between SPI and HMP was more stable at pH 3.0, and the Tp value increased from 145.1 to 155.21 °C.
format Article
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institution OA Journals
issn 2590-1575
language English
publishDate 2025-04-01
publisher Elsevier
record_format Article
series Food Chemistry: X
spelling doaj-art-1d99d0cd255a42a7b83de455f741051d2025-08-20T02:30:31ZengElsevierFood Chemistry: X2590-15752025-04-012710250010.1016/j.fochx.2025.102500Mechanistic investigation of a soybean protein isolate–soyasaponin–pectin composite system on soyasaponin bitterness in an acidic environmentLijie Zhu0Yingyan Li1He Li2Dayun Zhao3Xinqi Liu4Qian Shen5Qi Zhang6Qingyun Lv7Xiuying Liu8Wenping Ding9Key Laboratory for Deep Processing of Major Grain and Oil, Ministry of Education; School of Food Science and Engineering, Wuhan Polytechnic University, Wuhan, Hubei 430028, ChinaChina Food Flavor and Nutrition Health Innovation Center, Beijing Technology and Business University, Beijing 100048, China; College of Food Science and Technology, Bohai University; Jinzhou, Liaoning 121013, ChinaChina Food Flavor and Nutrition Health Innovation Center, Beijing Technology and Business University, Beijing 100048, ChinaSchool of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, ChinaChina Food Flavor and Nutrition Health Innovation Center, Beijing Technology and Business University, Beijing 100048, ChinaKey Laboratory for Deep Processing of Major Grain and Oil, Ministry of Education; School of Food Science and Engineering, Wuhan Polytechnic University, Wuhan, Hubei 430028, ChinaKey Laboratory for Deep Processing of Major Grain and Oil, Ministry of Education; School of Food Science and Engineering, Wuhan Polytechnic University, Wuhan, Hubei 430028, ChinaKey Laboratory for Deep Processing of Major Grain and Oil, Ministry of Education; School of Food Science and Engineering, Wuhan Polytechnic University, Wuhan, Hubei 430028, ChinaKey Laboratory for Deep Processing of Major Grain and Oil, Ministry of Education; School of Food Science and Engineering, Wuhan Polytechnic University, Wuhan, Hubei 430028, China; Corresponding authors at: School of Food Science and Engineering, Wuhan Polytechnic University, 36 Huanhuzhong Road, Dongxihu District, Wuhan, Hubei 430028, China.Key Laboratory for Deep Processing of Major Grain and Oil, Ministry of Education; School of Food Science and Engineering, Wuhan Polytechnic University, Wuhan, Hubei 430028, China; Corresponding authors at: School of Food Science and Engineering, Wuhan Polytechnic University, 36 Huanhuzhong Road, Dongxihu District, Wuhan, Hubei 430028, China.Herein, a soybean protein isolate–soyasaponin (SPI–Ssa) composite system was constructed to mimic a complex, multifaceted soybean product system. This system was stabilized via the addition of high-methoxy pectin (HMP) and low-methoxy pectin (LMP), and the bitter taste of Ssa was modulated. HMP and LMP were compared using sensory evaluation and an electronic tongue assay, revealing that HMP was more effective in suppressing bitterness. Moreover, the interfacial properties and structural characteristics of the SPI–Ssa–HMP composite system were determined at different pH values, and the effect of composite coalescence on the adsorption of Ssa in acidic and neutral environments was investigated. The network structure formed between SPI and HMP was determined by differential scanning isothermal titration calorimetry and Fourier transform infrared spectroscopy. The formation of the network structure between SPI and HMP was more stable at pH 3.0, and the Tp value increased from 145.1 to 155.21 °C.http://www.sciencedirect.com/science/article/pii/S2590157525003475High-methoxy pectinSoybean isolate proteinSoyasaponinBitternessAcidic environment
spellingShingle Lijie Zhu
Yingyan Li
He Li
Dayun Zhao
Xinqi Liu
Qian Shen
Qi Zhang
Qingyun Lv
Xiuying Liu
Wenping Ding
Mechanistic investigation of a soybean protein isolate–soyasaponin–pectin composite system on soyasaponin bitterness in an acidic environment
Food Chemistry: X
High-methoxy pectin
Soybean isolate protein
Soyasaponin
Bitterness
Acidic environment
title Mechanistic investigation of a soybean protein isolate–soyasaponin–pectin composite system on soyasaponin bitterness in an acidic environment
title_full Mechanistic investigation of a soybean protein isolate–soyasaponin–pectin composite system on soyasaponin bitterness in an acidic environment
title_fullStr Mechanistic investigation of a soybean protein isolate–soyasaponin–pectin composite system on soyasaponin bitterness in an acidic environment
title_full_unstemmed Mechanistic investigation of a soybean protein isolate–soyasaponin–pectin composite system on soyasaponin bitterness in an acidic environment
title_short Mechanistic investigation of a soybean protein isolate–soyasaponin–pectin composite system on soyasaponin bitterness in an acidic environment
title_sort mechanistic investigation of a soybean protein isolate soyasaponin pectin composite system on soyasaponin bitterness in an acidic environment
topic High-methoxy pectin
Soybean isolate protein
Soyasaponin
Bitterness
Acidic environment
url http://www.sciencedirect.com/science/article/pii/S2590157525003475
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