The effect of expired orthodontic bonding material on primary human gingival fibroblasts: In vitro study
AIM: Composite resins and bonding agents are indispensable in orthodontic practice, necessitating a thorough understanding of their cytotoxic effects, particularly when expired. This study aimed to evaluate the influence of expired composite and bonding materials on human gingival fibroblasts (hGFs)...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2025-03-01
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| Series: | Journal of Orthodontic Science |
| Subjects: | |
| Online Access: | https://journals.lww.com/10.4103/jos.jos_67_24 |
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| author | Maysaa Z. Khojah Abdel-Rahman Youssef Elham N. Alsahafi |
| author_facet | Maysaa Z. Khojah Abdel-Rahman Youssef Elham N. Alsahafi |
| author_sort | Maysaa Z. Khojah |
| collection | DOAJ |
| description | AIM:
Composite resins and bonding agents are indispensable in orthodontic practice, necessitating a thorough understanding of their cytotoxic effects, particularly when expired. This study aimed to evaluate the influence of expired composite and bonding materials on human gingival fibroblasts (hGFs).
MATERIALS AND METHODS:
Both expired and nonexpired composite resins and bonding agents were tested on hGFs using direct exposure methods. Viability assays, morphological evaluations, and wound healing assays were conducted at 24 and 72 hours post exposure.
RESULTS:
Exposure to both expired and nonexpired materials led to significant reductions in hGF viability and alterations in morphology. Wound healing assays demonstrated impaired migratory abilities of hGFs following exposure to these materials.
CONCLUSION:
The study highlights the importance of prudent material selection, handling, and monitoring in orthodontic practice to mitigate adverse effects on gingival tissues. Proper management of expired materials is crucial for cost-effectiveness, waste reduction, and patient safety. Further research into the long-term impacts of expired materials on gingival and periodontal health is imperative to ensure clinical treatment safety and efficacy. This investigation provides valuable insights into the biocompatibility of orthodontic bonding materials and emphasizes the necessity for continued vigilance in their usage to uphold patient welfare and treatment quality. |
| format | Article |
| id | doaj-art-1d952ec8fc774ae4b3d49eb33e319334 |
| institution | DOAJ |
| issn | 2278-1897 2278-0203 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Journal of Orthodontic Science |
| spelling | doaj-art-1d952ec8fc774ae4b3d49eb33e3193342025-08-20T03:07:54ZengWolters Kluwer Medknow PublicationsJournal of Orthodontic Science2278-18972278-02032025-03-011413310.4103/jos.jos_67_24The effect of expired orthodontic bonding material on primary human gingival fibroblasts: In vitro studyMaysaa Z. KhojahAbdel-Rahman YoussefElham N. AlsahafiAIM: Composite resins and bonding agents are indispensable in orthodontic practice, necessitating a thorough understanding of their cytotoxic effects, particularly when expired. This study aimed to evaluate the influence of expired composite and bonding materials on human gingival fibroblasts (hGFs). MATERIALS AND METHODS: Both expired and nonexpired composite resins and bonding agents were tested on hGFs using direct exposure methods. Viability assays, morphological evaluations, and wound healing assays were conducted at 24 and 72 hours post exposure. RESULTS: Exposure to both expired and nonexpired materials led to significant reductions in hGF viability and alterations in morphology. Wound healing assays demonstrated impaired migratory abilities of hGFs following exposure to these materials. CONCLUSION: The study highlights the importance of prudent material selection, handling, and monitoring in orthodontic practice to mitigate adverse effects on gingival tissues. Proper management of expired materials is crucial for cost-effectiveness, waste reduction, and patient safety. Further research into the long-term impacts of expired materials on gingival and periodontal health is imperative to ensure clinical treatment safety and efficacy. This investigation provides valuable insights into the biocompatibility of orthodontic bonding materials and emphasizes the necessity for continued vigilance in their usage to uphold patient welfare and treatment quality.https://journals.lww.com/10.4103/jos.jos_67_24bonding agentcompositeexpired materialorthodontics |
| spellingShingle | Maysaa Z. Khojah Abdel-Rahman Youssef Elham N. Alsahafi The effect of expired orthodontic bonding material on primary human gingival fibroblasts: In vitro study Journal of Orthodontic Science bonding agent composite expired material orthodontics |
| title | The effect of expired orthodontic bonding material on primary human gingival fibroblasts: In vitro study |
| title_full | The effect of expired orthodontic bonding material on primary human gingival fibroblasts: In vitro study |
| title_fullStr | The effect of expired orthodontic bonding material on primary human gingival fibroblasts: In vitro study |
| title_full_unstemmed | The effect of expired orthodontic bonding material on primary human gingival fibroblasts: In vitro study |
| title_short | The effect of expired orthodontic bonding material on primary human gingival fibroblasts: In vitro study |
| title_sort | effect of expired orthodontic bonding material on primary human gingival fibroblasts in vitro study |
| topic | bonding agent composite expired material orthodontics |
| url | https://journals.lww.com/10.4103/jos.jos_67_24 |
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