Simply cut out – Combining CRISPR/Cas9 RNPs and transiently selected telomere vectors for marker free-gene deletion in Trichoderma atroviride

Simply cut out – Combining CRISPR/Cas9 RNPs and transiently selected telomere vectors for marker free-gene deletion in Trichoderma atroviride

Trichoderma atroviride is a well-known mycoparasitic fungus widely used for the biological control of fungal plant pathogens. However, genetic manipulation in this organism remains challenging due to the limited availability of versatile and efficient molecular tools. Here, we present a CRISPR/Cas9-...

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Bibliographic Details
Main Authors: Mario Gründlinger, Chiara Ellensohn, Leo Drechsel, Ulrike Schreiner, Siebe Pierson, Clara Baldin, Susanne Zeilinger
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-07-01
Series:Frontiers in Genome Editing
Subjects:
Trichoderma atroviride
CRISPR/Cas9
telomeric vector
transient resistance
marker-free
Online Access:https://www.frontiersin.org/articles/10.3389/fgeed.2025.1623963/full
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https://www.frontiersin.org/articles/10.3389/fgeed.2025.1623963/full

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