Protocol for in vitro phase separation of N terminus of CIDEC proteins

Summary: Cell death-inducing DFF45-like effector C (CIDEC) condensation through N-terminal phase separation is a prerequisite for CIDEC-mediated lipid droplet (LD) fusion and lipid storage promotion. Here, we present a protocol for in vitro phase separation of the N terminus of CIDEC (CIDEC-N) prote...

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Bibliographic Details
Main Authors: Yiming Lu, Zanzan Zhu, Jianqin Wang, Xuchao Lyu, Peng Li, Feng-Jung Chen
Format: Article
Language:English
Published: Elsevier 2025-06-01
Series:STAR Protocols
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Online Access:http://www.sciencedirect.com/science/article/pii/S2666166725001856
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