3′ RACE Walking along a Large cDNA Employing Tiered Suppression PCR
Large genes present particular cloning difficulties, especially when expressed at relatively low levels. We describe a novel method, termed 3′ rapid amplification of cDNA ends (RACE) walking, for the rapid determination of unknown 3′ flanking sequence of a large cDNA. The technique is a derivative o...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2003-04-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/03344st04 |
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