Causal impact of gut microbiota on five liver diseases: insights from mendelian randomization and single-cell RNA sequencing
BackgroundLiver disease is among the top ten causes of death globally. With studies suggesting a link between gut microbiota (GM) and liver disease.MethodWe selected summary statistics data from the largest available whole-genome association study (n = 13,266) of GM by the MiBioGen consortium as the...
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Frontiers Media S.A.
2024-11-01
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| Series: | Frontiers in Genetics |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fgene.2024.1362139/full |
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| author | Na Li Na Li Xuanyi Chen Shuai Xiong Shuai Xiong Yuxin Cheng Jiali Deng Jiali Deng Junli Zhang Fei Yu Fei Yu Liyuan Hao Liyuan Hao Shenghao Li Shenghao Li Xiaoyu Hu |
| author_facet | Na Li Na Li Xuanyi Chen Shuai Xiong Shuai Xiong Yuxin Cheng Jiali Deng Jiali Deng Junli Zhang Fei Yu Fei Yu Liyuan Hao Liyuan Hao Shenghao Li Shenghao Li Xiaoyu Hu |
| author_sort | Na Li |
| collection | DOAJ |
| description | BackgroundLiver disease is among the top ten causes of death globally. With studies suggesting a link between gut microbiota (GM) and liver disease.MethodWe selected summary statistics data from the largest available whole-genome association study (n = 13,266) of GM by the MiBioGen consortium as the exposure, and obtained liver disease-related data from IEU Open GWAS and The NHGRI-EBI GWAS Catalog. A two-sample Mendelian Randomization (MR) analysis employing various methods, to establish the causal relationship between GM and five liver diseases. Meanwhile, single-cell RNA sequencing data were used to examine Prevotella-related genes expression under healthy and disease liver.ResultsThe IVW analysis indicate a causal relationship between GM and liver diseases, with Prevotella exhibiting a protective effect in all five liver diseases: Alcoholic liver disease (OR:0.81,95% confidence interval:0.66-1.00,PIVW = 0.0494); Cirrhosis (OR: 0.85,95% confidence interval: 0.73-0.99,PIVW = 0.0397); Hepatic failure, not elsewhere classified (OR:0.60,95% confidence interval:0.37-0.95,PIVW = 0.0305); Benign neoplasm:Liver (OR:0.39,95% confidence interval:0.2-0.75,PIVW = 0.0046); Malignant neoplasm of liver, primary (OR:0.41, 95% confidence interval:0.18-0.93,PIVW = 0.0334). The single-cell results suggest differential expression of Prevotella-related genes between liver disease patients and healthy individuals.ConclusionOur MR results show a causal relationship between the GM and liver disease. Prevotella displays a notable protective effect. This finding may enhance the precision of GM-based therapies and offer new insights for clinical research. |
| format | Article |
| id | doaj-art-1c6f7441b83046598ae9e14d55c56d6c |
| institution | Kabale University |
| issn | 1664-8021 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Genetics |
| spelling | doaj-art-1c6f7441b83046598ae9e14d55c56d6c2024-11-11T06:10:40ZengFrontiers Media S.A.Frontiers in Genetics1664-80212024-11-011510.3389/fgene.2024.13621391362139Causal impact of gut microbiota on five liver diseases: insights from mendelian randomization and single-cell RNA sequencingNa Li0Na Li1Xuanyi Chen2Shuai Xiong3Shuai Xiong4Yuxin Cheng5Jiali Deng6Jiali Deng7Junli Zhang8Fei Yu9Fei Yu10Liyuan Hao11Liyuan Hao12Shenghao Li13Shenghao Li14Xiaoyu Hu15Department of Clinical Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu, ChinaDepartment of Infectious Diseases, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, ChinaAcupunctureand Tuina College, Chengdu University of Traditional Chinese Medicine, Chengdu, ChinaDepartment of Clinical Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu, ChinaDepartment of Infectious Diseases, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, ChinaDepartment of Ophthalmology, Key Laboratory of Sichuan Province Ophthalmopathy Prevention and Cure and Visual Function Protection with TCM, Chengdu, Sichuan, ChinaDepartment of Clinical Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu, ChinaDepartment of Infectious Diseases, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, ChinaDepartment of Infectious Diseases, Jiangsu Province Hospital of Chinese Medicine, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, Jiangsu, ChinaDepartment of Clinical Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu, ChinaDepartment of Infectious Diseases, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, ChinaDepartment of Clinical Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu, ChinaDepartment of Infectious Diseases, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, ChinaDepartment of Clinical Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu, ChinaDepartment of Infectious Diseases, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, ChinaDepartment of Infectious Diseases, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, ChinaBackgroundLiver disease is among the top ten causes of death globally. With studies suggesting a link between gut microbiota (GM) and liver disease.MethodWe selected summary statistics data from the largest available whole-genome association study (n = 13,266) of GM by the MiBioGen consortium as the exposure, and obtained liver disease-related data from IEU Open GWAS and The NHGRI-EBI GWAS Catalog. A two-sample Mendelian Randomization (MR) analysis employing various methods, to establish the causal relationship between GM and five liver diseases. Meanwhile, single-cell RNA sequencing data were used to examine Prevotella-related genes expression under healthy and disease liver.ResultsThe IVW analysis indicate a causal relationship between GM and liver diseases, with Prevotella exhibiting a protective effect in all five liver diseases: Alcoholic liver disease (OR:0.81,95% confidence interval:0.66-1.00,PIVW = 0.0494); Cirrhosis (OR: 0.85,95% confidence interval: 0.73-0.99,PIVW = 0.0397); Hepatic failure, not elsewhere classified (OR:0.60,95% confidence interval:0.37-0.95,PIVW = 0.0305); Benign neoplasm:Liver (OR:0.39,95% confidence interval:0.2-0.75,PIVW = 0.0046); Malignant neoplasm of liver, primary (OR:0.41, 95% confidence interval:0.18-0.93,PIVW = 0.0334). The single-cell results suggest differential expression of Prevotella-related genes between liver disease patients and healthy individuals.ConclusionOur MR results show a causal relationship between the GM and liver disease. Prevotella displays a notable protective effect. This finding may enhance the precision of GM-based therapies and offer new insights for clinical research.https://www.frontiersin.org/articles/10.3389/fgene.2024.1362139/fullgut microbiotafive liver diseasesPrevotellaMendelian randomizationSNPs |
| spellingShingle | Na Li Na Li Xuanyi Chen Shuai Xiong Shuai Xiong Yuxin Cheng Jiali Deng Jiali Deng Junli Zhang Fei Yu Fei Yu Liyuan Hao Liyuan Hao Shenghao Li Shenghao Li Xiaoyu Hu Causal impact of gut microbiota on five liver diseases: insights from mendelian randomization and single-cell RNA sequencing Frontiers in Genetics gut microbiota five liver diseases Prevotella Mendelian randomization SNPs |
| title | Causal impact of gut microbiota on five liver diseases: insights from mendelian randomization and single-cell RNA sequencing |
| title_full | Causal impact of gut microbiota on five liver diseases: insights from mendelian randomization and single-cell RNA sequencing |
| title_fullStr | Causal impact of gut microbiota on five liver diseases: insights from mendelian randomization and single-cell RNA sequencing |
| title_full_unstemmed | Causal impact of gut microbiota on five liver diseases: insights from mendelian randomization and single-cell RNA sequencing |
| title_short | Causal impact of gut microbiota on five liver diseases: insights from mendelian randomization and single-cell RNA sequencing |
| title_sort | causal impact of gut microbiota on five liver diseases insights from mendelian randomization and single cell rna sequencing |
| topic | gut microbiota five liver diseases Prevotella Mendelian randomization SNPs |
| url | https://www.frontiersin.org/articles/10.3389/fgene.2024.1362139/full |
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