The Effects of the Combined Co-Expression of GroEL/ES and Trigger Factor Chaperones on Orthopoxvirus Phospholipase F13 Production in <i>E. coli</i>

The Effects of the Combined Co-Expression of GroEL/ES and Trigger Factor Chaperones on Orthopoxvirus Phospholipase F13 Production in <i>E. coli</i>

Heterologous protein expression often faces significant challenges, particularly when the target protein has posttranslational modifications, is toxic, or is prone to misfolding. These issues can result in low expression levels, aggregation, or even cell death. Such problems are exemplified by the e...

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Bibliographic Details
Main Authors: Iuliia A. Merkuleva, Vladimir N. Nikitin, Tatyana D. Belaya, Egor. A. Mustaev, Dmitriy N. Shcherbakov
Format: Article
Language:English
Published: MDPI AG 2024-12-01
Series:BioTech
Subjects:
orthopoxviruses
monkeypox virus
phospholipase F13
p37
ST-246
NIOCH-14
Online Access:https://www.mdpi.com/2673-6284/13/4/57
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