Specific Detection of African Swine Fever Virus Variants: Novel Quadplex Real-Time PCR Assay with Internal Control

Specific Detection of African Swine Fever Virus Variants: Novel Quadplex Real-Time PCR Assay with Internal Control

African swine fever (ASF), a highly contagious and lethal viral disease, continues to devastate the global swine industry. The emergence of ASF virus (ASFV) variants with varying genomic deletions poses significant challenges for ASF control. This study presents a novel, sensitive, and reliable quad...

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Bibliographic Details
Main Authors: Lihua Wang, Yuzhen Li, Xirui Zhang, Rachel Madera, Homer Pantua, Aidan Craig, Nina Muro, Danqin Li, Jamie Retallick, Franco Matias Ferreyra, Quang Lam Truong, Lan Thi Nguyen, Jishu Shi
Format: Article
Language:English
Published: MDPI AG 2025-03-01
Series:Microorganisms
Subjects:
African swine fever
variants
real-time PCR
internal control
sensitive
specific
Online Access:https://www.mdpi.com/2076-2607/13/3/615
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