Application of 2D polymerase chain reaction for single-tube detection of high-risk human papillomaviruses

Application of 2D polymerase chain reaction for single-tube detection of high-risk human papillomaviruses

PurposeThe persistent infection of high-risk HPV (HR-HPV) is intricately linked to the onset and progression of cervical cancer. This research endeavored to develop a high-throughput 2D PCR method for closed-tube genotyping of 11 HR-HPVs.MethodsBase-quenched probes were specifically designed for FAM...

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Bibliographic Details
Main Authors:	Weifeng Wang, Min Jiang, Ying Liu, Xuan Wu, Yan Chen, Xiaoyun Zhang, Weiwei Liu
Format:	Article
Language:	English
Published:	Frontiers Media S.A. 2025-01-01
Series:	Frontiers in Microbiology
Subjects:	human papillomavirus two-dimensional PCR cervical cancer melting temperature high-throughput
Online Access:	https://www.frontiersin.org/articles/10.3389/fmicb.2025.1528094/full
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