Structural bioinformatics approaches for predicting novel drug targets in hepatitis C virus proteins: a comprehensive analysis

Abstract This study employs structural bioinformatics approaches to identify and evaluate potential drug targets within the Hepatitis C virus (HCV) proteome. Through integration of homology modeling, molecular docking, and molecular dynamics simulations, we analyzed the structural features and drugg...

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Main Authors: Miao Qu, Mingzhu Gao, Xisheng Sang, Miao Yu, Zihe Guan, Weizhi Chang
Format: Article
Language:English
Published: Nature Portfolio 2025-07-01
Series:Scientific Reports
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Online Access:https://doi.org/10.1038/s41598-025-12563-w
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author Miao Qu
Mingzhu Gao
Xisheng Sang
Miao Yu
Zihe Guan
Weizhi Chang
author_facet Miao Qu
Mingzhu Gao
Xisheng Sang
Miao Yu
Zihe Guan
Weizhi Chang
author_sort Miao Qu
collection DOAJ
description Abstract This study employs structural bioinformatics approaches to identify and evaluate potential drug targets within the Hepatitis C virus (HCV) proteome. Through integration of homology modeling, molecular docking, and molecular dynamics simulations, we analyzed the structural features and druggability of key HCV proteins. The research focused on predicting binding sites, evaluating protein-ligand interactions, and assessing the therapeutic potential of identified targets. Our findings revealed promising drug targets including the NS3 protease, NS5B polymerase, core protein, and NS5A, with detailed characterization of their binding pockets and interaction patterns. The study provides structural insights for rational drug design against HCV and demonstrates the utility of computational approaches in antiviral drug discovery. While experimental validation is needed, these results contribute to the development of novel anti-HCV therapeutics and highlight potential strategies for targeted intervention.
format Article
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institution Kabale University
issn 2045-2322
language English
publishDate 2025-07-01
publisher Nature Portfolio
record_format Article
series Scientific Reports
spelling doaj-art-19f46420dfe5427484d96aae83aada7b2025-08-20T04:03:01ZengNature PortfolioScientific Reports2045-23222025-07-0115111210.1038/s41598-025-12563-wStructural bioinformatics approaches for predicting novel drug targets in hepatitis C virus proteins: a comprehensive analysisMiao Qu0Mingzhu Gao1Xisheng Sang2Miao Yu3Zihe Guan4Weizhi Chang5School of basic Medicine, Heilongjiang University of Chinese MedicineSchool of basic Medicine, Heilongjiang University of Chinese MedicineSchool of basic Medicine, Heilongjiang University of Chinese MedicineSchool of basic Medicine, Heilongjiang University of Chinese MedicineSchool of basic Medicine, Heilongjiang University of Chinese MedicineSchool of basic Medicine, Heilongjiang University of Chinese MedicineAbstract This study employs structural bioinformatics approaches to identify and evaluate potential drug targets within the Hepatitis C virus (HCV) proteome. Through integration of homology modeling, molecular docking, and molecular dynamics simulations, we analyzed the structural features and druggability of key HCV proteins. The research focused on predicting binding sites, evaluating protein-ligand interactions, and assessing the therapeutic potential of identified targets. Our findings revealed promising drug targets including the NS3 protease, NS5B polymerase, core protein, and NS5A, with detailed characterization of their binding pockets and interaction patterns. The study provides structural insights for rational drug design against HCV and demonstrates the utility of computational approaches in antiviral drug discovery. While experimental validation is needed, these results contribute to the development of novel anti-HCV therapeutics and highlight potential strategies for targeted intervention.https://doi.org/10.1038/s41598-025-12563-wHepatitis C virusStructural bioinformaticsDrug targetsMolecular dockingAntiviral therapeuticsProtein modeling
spellingShingle Miao Qu
Mingzhu Gao
Xisheng Sang
Miao Yu
Zihe Guan
Weizhi Chang
Structural bioinformatics approaches for predicting novel drug targets in hepatitis C virus proteins: a comprehensive analysis
Scientific Reports
Hepatitis C virus
Structural bioinformatics
Drug targets
Molecular docking
Antiviral therapeutics
Protein modeling
title Structural bioinformatics approaches for predicting novel drug targets in hepatitis C virus proteins: a comprehensive analysis
title_full Structural bioinformatics approaches for predicting novel drug targets in hepatitis C virus proteins: a comprehensive analysis
title_fullStr Structural bioinformatics approaches for predicting novel drug targets in hepatitis C virus proteins: a comprehensive analysis
title_full_unstemmed Structural bioinformatics approaches for predicting novel drug targets in hepatitis C virus proteins: a comprehensive analysis
title_short Structural bioinformatics approaches for predicting novel drug targets in hepatitis C virus proteins: a comprehensive analysis
title_sort structural bioinformatics approaches for predicting novel drug targets in hepatitis c virus proteins a comprehensive analysis
topic Hepatitis C virus
Structural bioinformatics
Drug targets
Molecular docking
Antiviral therapeutics
Protein modeling
url https://doi.org/10.1038/s41598-025-12563-w
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