Continuous cell lines derived from the Asian citrus psyllid, Diaphorina citri, harbor viruses and Wolbachia
Abstract The Asian citrus psyllid, Diaphorina citri Kuwayama (Hemiptera: Liviidae), is a major pest of global citriculture. In the Americas and in Asia, D. citri vectors the phloem-limited bacterium, Candidatus Liberibacter asiaticus (CLas), which causes the fatal citrus disease huanglongbing, or ci...
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Nature Portfolio
2025-01-01
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| Online Access: | https://doi.org/10.1038/s41598-024-83671-2 |
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| author | Ke Wu Emily D. Vu Saptarshi Ghosh Ruchir Mishra Bryony C. Bonning |
| author_facet | Ke Wu Emily D. Vu Saptarshi Ghosh Ruchir Mishra Bryony C. Bonning |
| author_sort | Ke Wu |
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| description | Abstract The Asian citrus psyllid, Diaphorina citri Kuwayama (Hemiptera: Liviidae), is a major pest of global citriculture. In the Americas and in Asia, D. citri vectors the phloem-limited bacterium, Candidatus Liberibacter asiaticus (CLas), which causes the fatal citrus disease huanglongbing, or citrus greening. Cell lines derived from D. citri can provide insight into both the basic biology of this pest and D. citri-associated pathogens including CLas. We previously identified CLG#2 as the optimal medium for long-term growth of D. citri primary cell cultures. Here we report on the establishment and characterization of three continuous D. citri cell lines, Dici1, Dici3, and Dici5, that have been passaged for > 40 times. Based on morphological and transcriptomic data, the Dici1 and Dici3 cell lines include undifferentiated and neurogenic progenitor cells. Dici1 and Dici5 are infected with Wolbachia. Both Dici1 and Dici5 are infected with D. citri reovirus, and Dici5 is also infected with D. citri-associated C virus. Dici3 is free of both Wolbachia and virus infection. These cell lines provide an ideal platform for the study of inter-microbial relationships as well as microbe interaction with host insect cells. |
| format | Article |
| id | doaj-art-1981196b28844e07975daa508baf048c |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
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| spelling | doaj-art-1981196b28844e07975daa508baf048c2025-01-05T12:23:01ZengNature PortfolioScientific Reports2045-23222025-01-0115111310.1038/s41598-024-83671-2Continuous cell lines derived from the Asian citrus psyllid, Diaphorina citri, harbor viruses and WolbachiaKe Wu0Emily D. Vu1Saptarshi Ghosh2Ruchir Mishra3Bryony C. Bonning4Department of Entomology and Nematology, University of FloridaDepartment of Entomology and Nematology, University of FloridaDepartment of Entomology and Nematology, University of FloridaDepartment of Entomology and Nematology, University of FloridaDepartment of Entomology and Nematology, University of FloridaAbstract The Asian citrus psyllid, Diaphorina citri Kuwayama (Hemiptera: Liviidae), is a major pest of global citriculture. In the Americas and in Asia, D. citri vectors the phloem-limited bacterium, Candidatus Liberibacter asiaticus (CLas), which causes the fatal citrus disease huanglongbing, or citrus greening. Cell lines derived from D. citri can provide insight into both the basic biology of this pest and D. citri-associated pathogens including CLas. We previously identified CLG#2 as the optimal medium for long-term growth of D. citri primary cell cultures. Here we report on the establishment and characterization of three continuous D. citri cell lines, Dici1, Dici3, and Dici5, that have been passaged for > 40 times. Based on morphological and transcriptomic data, the Dici1 and Dici3 cell lines include undifferentiated and neurogenic progenitor cells. Dici1 and Dici5 are infected with Wolbachia. Both Dici1 and Dici5 are infected with D. citri reovirus, and Dici5 is also infected with D. citri-associated C virus. Dici3 is free of both Wolbachia and virus infection. These cell lines provide an ideal platform for the study of inter-microbial relationships as well as microbe interaction with host insect cells.https://doi.org/10.1038/s41598-024-83671-2Diaphorina citriAsian citrus psyllidCitrus greening diseaseInsect cell lineTranscriptomeWolbachia |
| spellingShingle | Ke Wu Emily D. Vu Saptarshi Ghosh Ruchir Mishra Bryony C. Bonning Continuous cell lines derived from the Asian citrus psyllid, Diaphorina citri, harbor viruses and Wolbachia Scientific Reports Diaphorina citri Asian citrus psyllid Citrus greening disease Insect cell line Transcriptome Wolbachia |
| title | Continuous cell lines derived from the Asian citrus psyllid, Diaphorina citri, harbor viruses and Wolbachia |
| title_full | Continuous cell lines derived from the Asian citrus psyllid, Diaphorina citri, harbor viruses and Wolbachia |
| title_fullStr | Continuous cell lines derived from the Asian citrus psyllid, Diaphorina citri, harbor viruses and Wolbachia |
| title_full_unstemmed | Continuous cell lines derived from the Asian citrus psyllid, Diaphorina citri, harbor viruses and Wolbachia |
| title_short | Continuous cell lines derived from the Asian citrus psyllid, Diaphorina citri, harbor viruses and Wolbachia |
| title_sort | continuous cell lines derived from the asian citrus psyllid diaphorina citri harbor viruses and wolbachia |
| topic | Diaphorina citri Asian citrus psyllid Citrus greening disease Insect cell line Transcriptome Wolbachia |
| url | https://doi.org/10.1038/s41598-024-83671-2 |
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