The effects of RT-qPCR standards on reproducibility and comparability in monitoring SARS-CoV-2 levels in wastewater
Abstract Reverse transcription-quantitative PCR (RT-qPCR) is widely used for monitoring viruses, including severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), in wastewater. Various materials, including plasmid DNA, synthetic nucleic acids, PCR amplicons, genomic DNA, and cDNA, are current...
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| Main Authors: | Aapo Juutinen, Ananda Tiwari, Anna-Maria Hokajärvi, Oskari Luomala, Aleksi Kolehmainen, Eveliina Nurmi, Elisa Salmivirta, Tarja Pitkänen, Anssi Lipponen |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2024-10-01
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| Series: | Scientific Reports |
| Subjects: | |
| Online Access: | https://doi.org/10.1038/s41598-024-77155-6 |
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