The effects of RT-qPCR standards on reproducibility and comparability in monitoring SARS-CoV-2 levels in wastewater

The effects of RT-qPCR standards on reproducibility and comparability in monitoring SARS-CoV-2 levels in wastewater

Abstract Reverse transcription-quantitative PCR (RT-qPCR) is widely used for monitoring viruses, including severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), in wastewater. Various materials, including plasmid DNA, synthetic nucleic acids, PCR amplicons, genomic DNA, and cDNA, are current...

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Main Authors: Aapo Juutinen, Ananda Tiwari, Anna-Maria Hokajärvi, Oskari Luomala, Aleksi Kolehmainen, Eveliina Nurmi, Elisa Salmivirta, Tarja Pitkänen, Anssi Lipponen
Format: Article
Language:English
Published: Nature Portfolio 2024-10-01
Series:Scientific Reports
Subjects:
Standard control
SARS-CoV-2
Wastewater surveillance
Wastewater-based epidemiology
Real-time quantitative PCR
Online Access:https://doi.org/10.1038/s41598-024-77155-6
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https://doi.org/10.1038/s41598-024-77155-6

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