Directed assembly of DNA molecules via simultaneous ligation and digestion

DNA ligation is a routine laboratory practice, yet the yield of the desired product is often very low due to competing off-pathway reactions. The sensitivity of subsequent manipulations (e.g., selection via bacterial transformation) often obviates the need for a high yield of correctly ligated produ...

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Bibliographic Details
Main Authors: Gregory J. Cost, Nicholas R. Cozzarelli
Format: Article
Language:English
Published: Taylor & Francis Group 2007-01-01
Series:BioTechniques
Online Access:https://www.future-science.com/doi/10.2144/000112283
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