Effect of Xylooligosaccharides on the Metabolic Activity of <i>Lactiplantibacillus plantarum</i> S61: Production of Bioactive Metabolites with Antioxidant and Antimicrobial Properties
This study aimed to characterize the fermentation of commercial xylooligosaccharide (XOS) using <i>Lactiplantibacillus plantarum</i> S61. XOS was utilized as the main carbon source in a modified MRS medium (MRSm) at concentrations of 1%, 2%, 4%, and 6% (<i>w</i>/<i>v<...
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MDPI AG
2025-03-01
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| author | Meryem Idrissi Yahyaoui Nour Eddine Bentouhami Sara Moumnassi Amine Elbouzidi Mohamed Taibi Doha Berraaouan Reda Bellaouchi Bassem Jaouadi Abdelkarim Abousalham Ennouamane Saalaoui Abdeslam Asehraou |
| author_facet | Meryem Idrissi Yahyaoui Nour Eddine Bentouhami Sara Moumnassi Amine Elbouzidi Mohamed Taibi Doha Berraaouan Reda Bellaouchi Bassem Jaouadi Abdelkarim Abousalham Ennouamane Saalaoui Abdeslam Asehraou |
| author_sort | Meryem Idrissi Yahyaoui |
| collection | DOAJ |
| description | This study aimed to characterize the fermentation of commercial xylooligosaccharide (XOS) using <i>Lactiplantibacillus plantarum</i> S61. XOS was utilized as the main carbon source in a modified MRS medium (MRSm) at concentrations of 1%, 2%, 4%, and 6% (<i>w</i>/<i>v</i>). The growth of <i>L. plantarum</i> S61 was tracked daily over a week and compared to a control MRS medium using glucose as the main carbon source. The pH, total free acidity, and biomass were analyzed during fermentation, and the resulting fermentation product was analyzed for its content of fatty acids, total polyphenols, and the production of antioxidant and antimicrobial metabolites. The findings revealed that <i>L. plantarum</i> S61 thrived in MRSm, particularly at a 6% XOS concentration, which was identified as the best condition among the four tested concentrations (1%, 2%, 4%, and 6%). The fermentation of XOS by <i>L. plantarum</i> S61 significantly increased the total phenolic content and antioxidant activity compared to glucose, with the highest phenolic content observed at 6% XOS (6.70 ± 0.01 mg GAE/g). The strains produced various organic acids including lactic, malic, and oxalic acids from glucose and XOS. Notably, the strains yielded a broader range of organic acids with higher contents in the presence of XOS, leading to enhanced antifungal and antibacterial activities compared to glucose. Significant antibacterial activity was observed against Gram-negative bacteria, particularly <i>Salmonella enterica</i>, with inhibition zones of 20.25 mm and 11.75 mm for XOS (5% and 6%) and glucose, respectively. For Gram-positive bacteria, <i>S. aureus</i> and <i>M. luteus</i> exhibited inhibition zones of 20.01 mm for XOS 6% and 10.25 mm for glucose. In terms of antifungal activity, the strongest inhibition was observed against <i>Rhodotorula glutinis</i>, with inhibition zones of 26.00 mm for XOS 6% and 20.04 mm for glucose. The fermentation product of XOS by <i>L. plantarum</i> S61 showed significant inhibitory effects against pathogenic bacteria and yeasts, suggesting its potential application in the manufacturing of preservatives with antifungal properties and pharmaceutical products targeting pathogenic and spoilage yeasts. |
| format | Article |
| id | doaj-art-186fa594998d456b91f398e18185cd87 |
| institution | DOAJ |
| issn | 2674-1334 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Bacteria |
| spelling | doaj-art-186fa594998d456b91f398e18185cd872025-08-20T02:42:38ZengMDPI AGBacteria2674-13342025-03-01411410.3390/bacteria4010014Effect of Xylooligosaccharides on the Metabolic Activity of <i>Lactiplantibacillus plantarum</i> S61: Production of Bioactive Metabolites with Antioxidant and Antimicrobial PropertiesMeryem Idrissi Yahyaoui0Nour Eddine Bentouhami1Sara Moumnassi2Amine Elbouzidi3Mohamed Taibi4Doha Berraaouan5Reda Bellaouchi6Bassem Jaouadi7Abdelkarim Abousalham8Ennouamane Saalaoui9Abdeslam Asehraou10Laboratory of Bioresources, Biotechnology, Ethnopharmacology and Health, Faculty of Sciences, University Mohammed Premier, Oujda 60000, MoroccoLaboratory of Bioresources, Biotechnology, Ethnopharmacology and Health, Faculty of Sciences, University Mohammed Premier, Oujda 60000, MoroccoLaboratory of Bioresources, Biotechnology, Ethnopharmacology and Health, Faculty of Sciences, University Mohammed Premier, Oujda 60000, MoroccoLaboratoire d’Amélioration des Productions Agricoles, Biotechnologie et Environnement (LAPABE), Faculté des Sciences, Université Mohammed Premier, Oujda 60000, MoroccoLaboratoire d’Amélioration des Productions Agricoles, Biotechnologie et Environnement (LAPABE), Faculté des Sciences, Université Mohammed Premier, Oujda 60000, MoroccoPhysical Chemistry of Natural Substances and Process Research Team, Laboratory of Applied Chemistry and Environment (LCAE-CPSUNAP), Faculty of Science, Mohammed 1st University, Oujda 60000, MoroccoLaboratory of Bioresources, Biotechnology, Ethnopharmacology and Health, Faculty of Sciences, University Mohammed Premier, Oujda 60000, MoroccoLaboratory of Microbial and Enzymatic Biotechnology and Biomolecules (LMEBB), Centre of Biotechnology of Sfax (CBS), University of Sfax (USF), Road of Sidi Mansour Km 6, P.O. Box 1177, Sfax 3018, TunisiaGénie Enzymatique, Membranes Biomimétiques et Assemblages Supramoléculaires (GEMBAS), Institut de Chimie et de Biochimie Moléculaires et Supramoléculaires (ICBMS), UMR 5246 CNRS, University Lyon 1, Bât Raulin, 43 Bd du 11 Novembre 1918, CEDEX, F-69622 Villeurbanne, FranceLaboratory of Bioresources, Biotechnology, Ethnopharmacology and Health, Faculty of Sciences, University Mohammed Premier, Oujda 60000, MoroccoLaboratory of Bioresources, Biotechnology, Ethnopharmacology and Health, Faculty of Sciences, University Mohammed Premier, Oujda 60000, MoroccoThis study aimed to characterize the fermentation of commercial xylooligosaccharide (XOS) using <i>Lactiplantibacillus plantarum</i> S61. XOS was utilized as the main carbon source in a modified MRS medium (MRSm) at concentrations of 1%, 2%, 4%, and 6% (<i>w</i>/<i>v</i>). The growth of <i>L. plantarum</i> S61 was tracked daily over a week and compared to a control MRS medium using glucose as the main carbon source. The pH, total free acidity, and biomass were analyzed during fermentation, and the resulting fermentation product was analyzed for its content of fatty acids, total polyphenols, and the production of antioxidant and antimicrobial metabolites. The findings revealed that <i>L. plantarum</i> S61 thrived in MRSm, particularly at a 6% XOS concentration, which was identified as the best condition among the four tested concentrations (1%, 2%, 4%, and 6%). The fermentation of XOS by <i>L. plantarum</i> S61 significantly increased the total phenolic content and antioxidant activity compared to glucose, with the highest phenolic content observed at 6% XOS (6.70 ± 0.01 mg GAE/g). The strains produced various organic acids including lactic, malic, and oxalic acids from glucose and XOS. Notably, the strains yielded a broader range of organic acids with higher contents in the presence of XOS, leading to enhanced antifungal and antibacterial activities compared to glucose. Significant antibacterial activity was observed against Gram-negative bacteria, particularly <i>Salmonella enterica</i>, with inhibition zones of 20.25 mm and 11.75 mm for XOS (5% and 6%) and glucose, respectively. For Gram-positive bacteria, <i>S. aureus</i> and <i>M. luteus</i> exhibited inhibition zones of 20.01 mm for XOS 6% and 10.25 mm for glucose. In terms of antifungal activity, the strongest inhibition was observed against <i>Rhodotorula glutinis</i>, with inhibition zones of 26.00 mm for XOS 6% and 20.04 mm for glucose. The fermentation product of XOS by <i>L. plantarum</i> S61 showed significant inhibitory effects against pathogenic bacteria and yeasts, suggesting its potential application in the manufacturing of preservatives with antifungal properties and pharmaceutical products targeting pathogenic and spoilage yeasts.https://www.mdpi.com/2674-1334/4/1/14xylooligosaccharideprebioticsprobiotics<i>L. plantarum</i>fermentation |
| spellingShingle | Meryem Idrissi Yahyaoui Nour Eddine Bentouhami Sara Moumnassi Amine Elbouzidi Mohamed Taibi Doha Berraaouan Reda Bellaouchi Bassem Jaouadi Abdelkarim Abousalham Ennouamane Saalaoui Abdeslam Asehraou Effect of Xylooligosaccharides on the Metabolic Activity of <i>Lactiplantibacillus plantarum</i> S61: Production of Bioactive Metabolites with Antioxidant and Antimicrobial Properties Bacteria xylooligosaccharide prebiotics probiotics <i>L. plantarum</i> fermentation |
| title | Effect of Xylooligosaccharides on the Metabolic Activity of <i>Lactiplantibacillus plantarum</i> S61: Production of Bioactive Metabolites with Antioxidant and Antimicrobial Properties |
| title_full | Effect of Xylooligosaccharides on the Metabolic Activity of <i>Lactiplantibacillus plantarum</i> S61: Production of Bioactive Metabolites with Antioxidant and Antimicrobial Properties |
| title_fullStr | Effect of Xylooligosaccharides on the Metabolic Activity of <i>Lactiplantibacillus plantarum</i> S61: Production of Bioactive Metabolites with Antioxidant and Antimicrobial Properties |
| title_full_unstemmed | Effect of Xylooligosaccharides on the Metabolic Activity of <i>Lactiplantibacillus plantarum</i> S61: Production of Bioactive Metabolites with Antioxidant and Antimicrobial Properties |
| title_short | Effect of Xylooligosaccharides on the Metabolic Activity of <i>Lactiplantibacillus plantarum</i> S61: Production of Bioactive Metabolites with Antioxidant and Antimicrobial Properties |
| title_sort | effect of xylooligosaccharides on the metabolic activity of i lactiplantibacillus plantarum i s61 production of bioactive metabolites with antioxidant and antimicrobial properties |
| topic | xylooligosaccharide prebiotics probiotics <i>L. plantarum</i> fermentation |
| url | https://www.mdpi.com/2674-1334/4/1/14 |
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