Evaluation of the Anti-Inflammatory Activity of Microwave Extracts of <i>Thymus algeriensis</i>: <i>In Vitro</i>, <i>In Vivo</i>, and <i>In Silico</i> Studies
The objective of this work is to study the anti-inflammatory effect <i>in vitro</i> and <i>in vivo</i> of microwave (MW) extracts of <i>Thymus algeriensis</i>. The <i>in vitro</i> study was performed by the human red blood cell protection test, while t...
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| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-04-01
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| Series: | Analytica |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2673-4532/6/2/16 |
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| Summary: | The objective of this work is to study the anti-inflammatory effect <i>in vitro</i> and <i>in vivo</i> of microwave (MW) extracts of <i>Thymus algeriensis</i>. The <i>in vitro</i> study was performed by the human red blood cell protection test, while the <i>in vivo</i> study used the carrageenan-induced rat paw edema model. The experimental results were confirmed by a molecular docking calculation. The results indicated that all the microwave extracts have a moderate anti-inflammatory effect, depending on their richness in phenolic compounds. Among the extracts studied, the one obtained at 100 °C for 15 min exhibited the most pronounced anti-inflammatory effect, with an inhibition of 78.52%, which is attributed to its high flavonoid content. In particular, the flavonoids naringin and catechin showed the best affinity for the target protein, with values of −10.3 kcal/mol and −9.2 kcal/mol, respectively, as well as low inhibition constants of 0.028 μM and 0.18 μM. These results indicate that these flavonoids generate interactions that enhance the stability of the target ligand–protein complex, thus contributing to the observed anti-inflammatory effect. |
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| ISSN: | 2673-4532 |