Cellular Programming and Reprogramming: Sculpting Cell Fate for the Production of Dopamine Neurons for Cell Therapy
Pluripotent stem cells are regarded as a promising cell source to obtain human dopamine neurons in sufficient amounts and purity for cell replacement therapy. Importantly, the success of clinical applications depends on our ability to steer pluripotent stem cells towards the right neuronal identity....
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2012/412040 |
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| _version_ | 1832548244949827584 |
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| author | Julio C. Aguila Eva Hedlund Rosario Sanchez-Pernaute |
| author_facet | Julio C. Aguila Eva Hedlund Rosario Sanchez-Pernaute |
| author_sort | Julio C. Aguila |
| collection | DOAJ |
| description | Pluripotent stem cells are regarded as a promising cell source to obtain human dopamine neurons in sufficient amounts and purity for cell replacement therapy. Importantly, the
success of clinical applications depends on our ability to steer pluripotent stem cells towards the right neuronal identity. In Parkinson disease, the loss of dopamine neurons is more pronounced in the ventrolateral population that projects to the sensorimotor striatum. Because synapses are highly specific, only neurons with this precise identity will contribute, upon transplantation, to the synaptic reconstruction of the dorsal striatum. Thus, understanding the developmental cell program of the mesostriatal dopamine neurons is critical for the identification of the extrinsic signals and cell-intrinsic factors that instruct and, ultimately, determine cell identity. Here, we review how extrinsic signals and transcription factors act together during development to shape midbrain cell fates. Further, we discuss how these same factors can be applied in vitro to induce, select, and reprogram cells to the mesostriatal dopamine fate. |
| format | Article |
| id | doaj-art-183bc62b28a64e9196f5ceb8a2f834f9 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-183bc62b28a64e9196f5ceb8a2f834f92025-02-03T06:15:11ZengWileyStem Cells International1687-966X1687-96782012-01-01201210.1155/2012/412040412040Cellular Programming and Reprogramming: Sculpting Cell Fate for the Production of Dopamine Neurons for Cell TherapyJulio C. Aguila0Eva Hedlund1Rosario Sanchez-Pernaute2Laboratory of Stem Cells and Neural Repair, Fundacion Inbiomed, Paseo Mikeletegi 81, 20009 San Sebastian, SpainDepartment of Neuroscience, Karolinska Institutet, Retzius Road 8, 17177 Stockholm, SwedenLaboratory of Stem Cells and Neural Repair, Fundacion Inbiomed, Paseo Mikeletegi 81, 20009 San Sebastian, SpainPluripotent stem cells are regarded as a promising cell source to obtain human dopamine neurons in sufficient amounts and purity for cell replacement therapy. Importantly, the success of clinical applications depends on our ability to steer pluripotent stem cells towards the right neuronal identity. In Parkinson disease, the loss of dopamine neurons is more pronounced in the ventrolateral population that projects to the sensorimotor striatum. Because synapses are highly specific, only neurons with this precise identity will contribute, upon transplantation, to the synaptic reconstruction of the dorsal striatum. Thus, understanding the developmental cell program of the mesostriatal dopamine neurons is critical for the identification of the extrinsic signals and cell-intrinsic factors that instruct and, ultimately, determine cell identity. Here, we review how extrinsic signals and transcription factors act together during development to shape midbrain cell fates. Further, we discuss how these same factors can be applied in vitro to induce, select, and reprogram cells to the mesostriatal dopamine fate.http://dx.doi.org/10.1155/2012/412040 |
| spellingShingle | Julio C. Aguila Eva Hedlund Rosario Sanchez-Pernaute Cellular Programming and Reprogramming: Sculpting Cell Fate for the Production of Dopamine Neurons for Cell Therapy Stem Cells International |
| title | Cellular Programming and Reprogramming: Sculpting Cell Fate for the Production of Dopamine Neurons for Cell Therapy |
| title_full | Cellular Programming and Reprogramming: Sculpting Cell Fate for the Production of Dopamine Neurons for Cell Therapy |
| title_fullStr | Cellular Programming and Reprogramming: Sculpting Cell Fate for the Production of Dopamine Neurons for Cell Therapy |
| title_full_unstemmed | Cellular Programming and Reprogramming: Sculpting Cell Fate for the Production of Dopamine Neurons for Cell Therapy |
| title_short | Cellular Programming and Reprogramming: Sculpting Cell Fate for the Production of Dopamine Neurons for Cell Therapy |
| title_sort | cellular programming and reprogramming sculpting cell fate for the production of dopamine neurons for cell therapy |
| url | http://dx.doi.org/10.1155/2012/412040 |
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