Mutational Insights into GyrA and GyrB Genes in Mycobacterium tuberculosis: A Genetic Basis for Fluoroquinolone Resistance in Multidrug-resistant Tuberculosis

The global tuberculosis (TB) epidemic is becoming progressively more complex due to the increasing prevalence of multidrug-resistant TB (MDR-TB), particularly with resistance to fluoroquinolones (FQs). This study focuses on identifying genetic mutations in the gyrA and gyrB genes of Mycobacterium tu...

Full description

Saved in:
Bibliographic Details
Main Authors: Anam Shafiq, Haris Manzoor Khan, Mohd. Shahid, Nazish Fatima, Mo Ahamad Khan
Format: Article
Language:English
Published: Journal of Pure and Applied Microbiology 2024-12-01
Series:Journal of Pure and Applied Microbiology
Subjects:
Online Access:https://microbiologyjournal.org/mutational-insights-into-gyra-and-gyrb-genes-in-mycobacterium-tuberculosis-a-genetic-basis-for-fluoroquinolone-resistance-in-multidrug-resistant-tuberculosis/
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1850246212921327616
author Anam Shafiq
Haris Manzoor Khan
Mohd. Shahid
Nazish Fatima
Mo Ahamad Khan
author_facet Anam Shafiq
Haris Manzoor Khan
Mohd. Shahid
Nazish Fatima
Mo Ahamad Khan
author_sort Anam Shafiq
collection DOAJ
description The global tuberculosis (TB) epidemic is becoming progressively more complex due to the increasing prevalence of multidrug-resistant TB (MDR-TB), particularly with resistance to fluoroquinolones (FQs). This study focuses on identifying genetic mutations in the gyrA and gyrB genes of Mycobacterium tuberculosis that drive FQ resistance. Sputum samples from suspected pulmonary TB patients were analyzed using PCR and sequencing to detect mutations within the quinolone resistance-determining regions (QRDR). The analysis revealed that mutations in gyrA, especially S95T, are prevalent and play a key role in FQ resistance. Additionally, less frequent mutations in gyrB, such as E501D and A533P, were also detected. These findings shed light on the molecular mechanisms contributing to FQ resistance in MDR-TB strains and underscore the need for enhanced diagnostic methods to identify resistance patterns more accurately. The insights gained from this research offer a foundation for improving TB treatment approaches and addressing the growing challenge of drug-resistant TB worldwide.
format Article
id doaj-art-17c3678aec814e388bf22557de1271ae
institution OA Journals
issn 0973-7510
2581-690X
language English
publishDate 2024-12-01
publisher Journal of Pure and Applied Microbiology
record_format Article
series Journal of Pure and Applied Microbiology
spelling doaj-art-17c3678aec814e388bf22557de1271ae2025-08-20T01:59:14ZengJournal of Pure and Applied MicrobiologyJournal of Pure and Applied Microbiology0973-75102581-690X2024-12-0118426512658https://doi.org/10.22207/JPAM.18.4.38Mutational Insights into GyrA and GyrB Genes in Mycobacterium tuberculosis: A Genetic Basis for Fluoroquinolone Resistance in Multidrug-resistant TuberculosisAnam Shafiqhttps://orcid.org/0000-0002-3469-1597Haris Manzoor Khanhttps://orcid.org/0009-0008-2269-0256Mohd. Shahidhttps://orcid.org/0000-0002-4684-3607Nazish Fatimahttps://orcid.org/0000-0003-3371-087XMo Ahamad Khanhttps://orcid.org/0000-0002-6866-0035The global tuberculosis (TB) epidemic is becoming progressively more complex due to the increasing prevalence of multidrug-resistant TB (MDR-TB), particularly with resistance to fluoroquinolones (FQs). This study focuses on identifying genetic mutations in the gyrA and gyrB genes of Mycobacterium tuberculosis that drive FQ resistance. Sputum samples from suspected pulmonary TB patients were analyzed using PCR and sequencing to detect mutations within the quinolone resistance-determining regions (QRDR). The analysis revealed that mutations in gyrA, especially S95T, are prevalent and play a key role in FQ resistance. Additionally, less frequent mutations in gyrB, such as E501D and A533P, were also detected. These findings shed light on the molecular mechanisms contributing to FQ resistance in MDR-TB strains and underscore the need for enhanced diagnostic methods to identify resistance patterns more accurately. The insights gained from this research offer a foundation for improving TB treatment approaches and addressing the growing challenge of drug-resistant TB worldwide.https://microbiologyjournal.org/mutational-insights-into-gyra-and-gyrb-genes-in-mycobacterium-tuberculosis-a-genetic-basis-for-fluoroquinolone-resistance-in-multidrug-resistant-tuberculosis/mdr-tbfluoroquinolone resistancegyra mutationsgyrb mutationsdiagnosissequence analysis
spellingShingle Anam Shafiq
Haris Manzoor Khan
Mohd. Shahid
Nazish Fatima
Mo Ahamad Khan
Mutational Insights into GyrA and GyrB Genes in Mycobacterium tuberculosis: A Genetic Basis for Fluoroquinolone Resistance in Multidrug-resistant Tuberculosis
Journal of Pure and Applied Microbiology
mdr-tb
fluoroquinolone resistance
gyra mutations
gyrb mutations
diagnosis
sequence analysis
title Mutational Insights into GyrA and GyrB Genes in Mycobacterium tuberculosis: A Genetic Basis for Fluoroquinolone Resistance in Multidrug-resistant Tuberculosis
title_full Mutational Insights into GyrA and GyrB Genes in Mycobacterium tuberculosis: A Genetic Basis for Fluoroquinolone Resistance in Multidrug-resistant Tuberculosis
title_fullStr Mutational Insights into GyrA and GyrB Genes in Mycobacterium tuberculosis: A Genetic Basis for Fluoroquinolone Resistance in Multidrug-resistant Tuberculosis
title_full_unstemmed Mutational Insights into GyrA and GyrB Genes in Mycobacterium tuberculosis: A Genetic Basis for Fluoroquinolone Resistance in Multidrug-resistant Tuberculosis
title_short Mutational Insights into GyrA and GyrB Genes in Mycobacterium tuberculosis: A Genetic Basis for Fluoroquinolone Resistance in Multidrug-resistant Tuberculosis
title_sort mutational insights into gyra and gyrb genes in mycobacterium tuberculosis a genetic basis for fluoroquinolone resistance in multidrug resistant tuberculosis
topic mdr-tb
fluoroquinolone resistance
gyra mutations
gyrb mutations
diagnosis
sequence analysis
url https://microbiologyjournal.org/mutational-insights-into-gyra-and-gyrb-genes-in-mycobacterium-tuberculosis-a-genetic-basis-for-fluoroquinolone-resistance-in-multidrug-resistant-tuberculosis/
work_keys_str_mv AT anamshafiq mutationalinsightsintogyraandgyrbgenesinmycobacteriumtuberculosisageneticbasisforfluoroquinoloneresistanceinmultidrugresistanttuberculosis
AT harismanzoorkhan mutationalinsightsintogyraandgyrbgenesinmycobacteriumtuberculosisageneticbasisforfluoroquinoloneresistanceinmultidrugresistanttuberculosis
AT mohdshahid mutationalinsightsintogyraandgyrbgenesinmycobacteriumtuberculosisageneticbasisforfluoroquinoloneresistanceinmultidrugresistanttuberculosis
AT nazishfatima mutationalinsightsintogyraandgyrbgenesinmycobacteriumtuberculosisageneticbasisforfluoroquinoloneresistanceinmultidrugresistanttuberculosis
AT moahamadkhan mutationalinsightsintogyraandgyrbgenesinmycobacteriumtuberculosisageneticbasisforfluoroquinoloneresistanceinmultidrugresistanttuberculosis