DNA polymerase actively and sequentially displaces single-stranded DNA-binding proteins

Abstract Single-stranded DNA-binding proteins (SSBs) protect transiently exposed ssDNA, yet how DNA polymerase (DNAp) displaces them during replication remains unclear. Using single-molecule force spectroscopy, dual-color imaging, and molecular dynamics simulations on bacteriophage T7 DNAp and SSB,...

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Bibliographic Details
Main Authors: Longfu Xu, Shikai Jin, Mia Urem, Seung-Joo Lee, Meindert H. Lamers, Xun Chen, Peter G. Wolynes, Gijs J. L. Wuite
Format: Article
Language:English
Published: Nature Portfolio 2025-08-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-025-62531-1
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