Effects of Grape Seed Proanthocyanidins on Growth Performance, Jejunal Antioxidant Capacity, Gut Microbial Diversity, and Metabolites in Kangle Chickens
This study examined the effects of dietary supplementation with grape seed proanthocyanidins (GSPs) on the growth performance, serum biochemistry, jejunal antioxidant capacity, and jejunal microbiota and metabolites in Chinese indigenous Kangle chicken. In this experiment, 120 female Kangle chickens...
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2025-05-01
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| author | Qianqian Wang Qingcan Fan Xue Yang Wei Hu Lucheng Zheng Lijun Zhou Jinmeng Shi Xingxu Zhao Yong Zhang |
| author_facet | Qianqian Wang Qingcan Fan Xue Yang Wei Hu Lucheng Zheng Lijun Zhou Jinmeng Shi Xingxu Zhao Yong Zhang |
| author_sort | Qianqian Wang |
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| description | This study examined the effects of dietary supplementation with grape seed proanthocyanidins (GSPs) on the growth performance, serum biochemistry, jejunal antioxidant capacity, and jejunal microbiota and metabolites in Chinese indigenous Kangle chicken. In this experiment, 120 female Kangle chickens aged 30 days old were randomly allocated into three treatment groups: a control group (CON) fed a standard diet and two experimental groups fed diets supplemented with 200 mg/kg (LGSP) or 400 mg/kg (HGSP) of GSPs. The experiment consisted of a 7-day adaptation period followed by a 30-day feeding trial. The results demonstrated that GSP supplementation did not significantly improve their average daily gain or feed efficiency. However, the HGSP group showed significant improvements in their liver and jejunal indices, a reduced jejunal crypt depth, and increased villus-height-to-crypt-depth ratios compared to these values in the CON group. Furthermore, the HGSP group also exhibited elevated concentrations of cholesterol in their serum. Additionally, the oxidative stress levels were probably reduced in the jejuna of the HGSP group, as evidenced by reduced malondialdehyde (MDA) contents. Although jejunal microbial diversity remained unchanged, the metabolomic analysis identified significant upregulation of jejunal metabolites, particularly those associated with free radical scavenging, protein nutrition, and bile acid metabolism, which would be beneficial for maintaining intestinal health. These findings indicate that supplementing their diet with 400 mg/kg of GSPs could improve the health of Kangle chickens, underscoring their potential as a functional feed additive in the production of indigenous Chinese chickens. |
| format | Article |
| id | doaj-art-1770572648ec4e09967b11a410ca2723 |
| institution | Kabale University |
| issn | 2076-2615 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | MDPI AG |
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| series | Animals |
| spelling | doaj-art-1770572648ec4e09967b11a410ca27232025-08-20T03:47:52ZengMDPI AGAnimals2076-26152025-05-011510148110.3390/ani15101481Effects of Grape Seed Proanthocyanidins on Growth Performance, Jejunal Antioxidant Capacity, Gut Microbial Diversity, and Metabolites in Kangle ChickensQianqian Wang0Qingcan Fan1Xue Yang2Wei Hu3Lucheng Zheng4Lijun Zhou5Jinmeng Shi6Xingxu Zhao7Yong Zhang8College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Life Science and Resources and Environment, Yichun University, Yichun 336000, ChinaCollege of Life Science and Resources and Environment, Yichun University, Yichun 336000, ChinaCollege of Life Science and Resources and Environment, Yichun University, Yichun 336000, ChinaCollege of Life Science and Resources and Environment, Yichun University, Yichun 336000, ChinaCollege of Life Science and Resources and Environment, Yichun University, Yichun 336000, ChinaCollege of Life Science and Resources and Environment, Yichun University, Yichun 336000, ChinaCollege of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, ChinaThis study examined the effects of dietary supplementation with grape seed proanthocyanidins (GSPs) on the growth performance, serum biochemistry, jejunal antioxidant capacity, and jejunal microbiota and metabolites in Chinese indigenous Kangle chicken. In this experiment, 120 female Kangle chickens aged 30 days old were randomly allocated into three treatment groups: a control group (CON) fed a standard diet and two experimental groups fed diets supplemented with 200 mg/kg (LGSP) or 400 mg/kg (HGSP) of GSPs. The experiment consisted of a 7-day adaptation period followed by a 30-day feeding trial. The results demonstrated that GSP supplementation did not significantly improve their average daily gain or feed efficiency. However, the HGSP group showed significant improvements in their liver and jejunal indices, a reduced jejunal crypt depth, and increased villus-height-to-crypt-depth ratios compared to these values in the CON group. Furthermore, the HGSP group also exhibited elevated concentrations of cholesterol in their serum. Additionally, the oxidative stress levels were probably reduced in the jejuna of the HGSP group, as evidenced by reduced malondialdehyde (MDA) contents. Although jejunal microbial diversity remained unchanged, the metabolomic analysis identified significant upregulation of jejunal metabolites, particularly those associated with free radical scavenging, protein nutrition, and bile acid metabolism, which would be beneficial for maintaining intestinal health. These findings indicate that supplementing their diet with 400 mg/kg of GSPs could improve the health of Kangle chickens, underscoring their potential as a functional feed additive in the production of indigenous Chinese chickens.https://www.mdpi.com/2076-2615/15/10/1481grape seed proanthocyanidinsintestinal morphologyoxidative stressgut microbiotametabolomic analysis |
| spellingShingle | Qianqian Wang Qingcan Fan Xue Yang Wei Hu Lucheng Zheng Lijun Zhou Jinmeng Shi Xingxu Zhao Yong Zhang Effects of Grape Seed Proanthocyanidins on Growth Performance, Jejunal Antioxidant Capacity, Gut Microbial Diversity, and Metabolites in Kangle Chickens Animals grape seed proanthocyanidins intestinal morphology oxidative stress gut microbiota metabolomic analysis |
| title | Effects of Grape Seed Proanthocyanidins on Growth Performance, Jejunal Antioxidant Capacity, Gut Microbial Diversity, and Metabolites in Kangle Chickens |
| title_full | Effects of Grape Seed Proanthocyanidins on Growth Performance, Jejunal Antioxidant Capacity, Gut Microbial Diversity, and Metabolites in Kangle Chickens |
| title_fullStr | Effects of Grape Seed Proanthocyanidins on Growth Performance, Jejunal Antioxidant Capacity, Gut Microbial Diversity, and Metabolites in Kangle Chickens |
| title_full_unstemmed | Effects of Grape Seed Proanthocyanidins on Growth Performance, Jejunal Antioxidant Capacity, Gut Microbial Diversity, and Metabolites in Kangle Chickens |
| title_short | Effects of Grape Seed Proanthocyanidins on Growth Performance, Jejunal Antioxidant Capacity, Gut Microbial Diversity, and Metabolites in Kangle Chickens |
| title_sort | effects of grape seed proanthocyanidins on growth performance jejunal antioxidant capacity gut microbial diversity and metabolites in kangle chickens |
| topic | grape seed proanthocyanidins intestinal morphology oxidative stress gut microbiota metabolomic analysis |
| url | https://www.mdpi.com/2076-2615/15/10/1481 |
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