Comparative study on the expression characteristics of transgenes inserted into the Gt(ROSA)26Sor and H11 loci in mice
The Gt(ROSA)26Sor (ROSA26) and H11 loci are commonly used as safe harbors for the construction of targeted transgenic mice. However, it remains unclear whether these two loci have distinct effects on transgene expression. In this study, we insert three differently colored fluorescent protein express...
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| Format: | Article |
| Language: | English |
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China Science Publishing & Media Ltd.
2024-05-01
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| Series: | Acta Biochimica et Biophysica Sinica |
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| Online Access: | https://www.sciengine.com/doi/10.3724/abbs.2024081 |
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| author | Yu Zhilan Shi Jiahao Zhang Jingyu Wu Youbing Shen Ruling Fei Jian |
| author_facet | Yu Zhilan Shi Jiahao Zhang Jingyu Wu Youbing Shen Ruling Fei Jian |
| author_sort | Yu Zhilan |
| collection | DOAJ |
| description | The Gt(ROSA)26Sor (ROSA26) and H11 loci are commonly used as safe harbors for the construction of targeted transgenic mice. However, it remains unclear whether these two loci have distinct effects on transgene expression. In this study, we insert three differently colored fluorescent protein expression cassettes (EGFP, tdTomato and mTagBFP2) driven by the CAG promoter into the ROSA26 and H11 loci. We generate five single-transgenic mouse models and a triple-transgenic mouse model expressing three distinct fluorescent proteins simultaneously. Our results reveal that the efficiency of transgene expression is greater at the ROSA26 locus with a reverse orientation relative to the transcription of the ROSA26 gene. In most tissues examined, the efficiency of transgene expression at the ROSA26 locus exceeds that at the H11 locus. Moreover, the expression profiles of identical transgenes display discrepancies across various tissues, and notably, substantial heterogeneity in transgene expression is discernible within cells of the same tissue. Our findings offer a valuable reference for the selection of safe harbors and strategies for the construction of transgenic mouse models. |
| format | Article |
| id | doaj-art-1701b06e25594f5d9393c647733bc2f1 |
| institution | OA Journals |
| issn | 1672-9145 |
| language | English |
| publishDate | 2024-05-01 |
| publisher | China Science Publishing & Media Ltd. |
| record_format | Article |
| series | Acta Biochimica et Biophysica Sinica |
| spelling | doaj-art-1701b06e25594f5d9393c647733bc2f12025-08-20T02:32:38ZengChina Science Publishing & Media Ltd.Acta Biochimica et Biophysica Sinica1672-91452024-05-01561687169810.3724/abbs.202408120d259ccComparative study on the expression characteristics of transgenes inserted into the Gt(ROSA)26Sor and H11 loci in miceYu Zhilan0Shi Jiahao1Zhang Jingyu2Wu Youbing3Shen Ruling4Fei Jian5["School of Life Science and Technology, Tongji University, Shanghai 200092, China"]["School of Life Science and Technology, Tongji University, Shanghai 200092, China"]["School of Life Science and Technology, Tongji University, Shanghai 200092, China"]["Shanghai Engineering Research Center for Model Organisms, SMOC, Shanghai 201203, China"]["Shanghai Laboratory Animal Research Center, Shanghai 201203, China"]["School of Life Science and Technology, Tongji University, Shanghai 200092, China","Shanghai Engineering Research Center for Model Organisms, SMOC, Shanghai 201203, China"]The Gt(ROSA)26Sor (ROSA26) and H11 loci are commonly used as safe harbors for the construction of targeted transgenic mice. However, it remains unclear whether these two loci have distinct effects on transgene expression. In this study, we insert three differently colored fluorescent protein expression cassettes (EGFP, tdTomato and mTagBFP2) driven by the CAG promoter into the ROSA26 and H11 loci. We generate five single-transgenic mouse models and a triple-transgenic mouse model expressing three distinct fluorescent proteins simultaneously. Our results reveal that the efficiency of transgene expression is greater at the ROSA26 locus with a reverse orientation relative to the transcription of the ROSA26 gene. In most tissues examined, the efficiency of transgene expression at the ROSA26 locus exceeds that at the H11 locus. Moreover, the expression profiles of identical transgenes display discrepancies across various tissues, and notably, substantial heterogeneity in transgene expression is discernible within cells of the same tissue. Our findings offer a valuable reference for the selection of safe harbors and strategies for the construction of transgenic mouse models.https://www.sciengine.com/doi/10.3724/abbs.2024081transgenic mouseknockin<italic>Gt(ROSA)26Sor</italic><italic>H11</italic>gene expression |
| spellingShingle | Yu Zhilan Shi Jiahao Zhang Jingyu Wu Youbing Shen Ruling Fei Jian Comparative study on the expression characteristics of transgenes inserted into the Gt(ROSA)26Sor and H11 loci in mice Acta Biochimica et Biophysica Sinica transgenic mouse knockin <italic>Gt(ROSA)26Sor</italic> <italic>H11</italic> gene expression |
| title | Comparative study on the expression characteristics of transgenes inserted into the Gt(ROSA)26Sor and H11 loci in mice |
| title_full | Comparative study on the expression characteristics of transgenes inserted into the Gt(ROSA)26Sor and H11 loci in mice |
| title_fullStr | Comparative study on the expression characteristics of transgenes inserted into the Gt(ROSA)26Sor and H11 loci in mice |
| title_full_unstemmed | Comparative study on the expression characteristics of transgenes inserted into the Gt(ROSA)26Sor and H11 loci in mice |
| title_short | Comparative study on the expression characteristics of transgenes inserted into the Gt(ROSA)26Sor and H11 loci in mice |
| title_sort | comparative study on the expression characteristics of transgenes inserted into the gt rosa 26sor and h11 loci in mice |
| topic | transgenic mouse knockin <italic>Gt(ROSA)26Sor</italic> <italic>H11</italic> gene expression |
| url | https://www.sciengine.com/doi/10.3724/abbs.2024081 |
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