γ-Aminobutyric acid alleviates litchi thaumatin-like protein-induced inflammation and reduces gut microbial translocation
Previous research reported litchi thaumatin-like protein (LcTLP) could lead to inflammation, which is a factor causing the adverse reactions after excessive intake of litchi. As a main amino acid in litchi pulp, γ-aminobutyric acid (GABA) was found with anti-inflammatory effect. Therefore, this stud...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Tsinghua University Press
2024-09-01
|
| Series: | Food Science and Human Wellness |
| Subjects: | |
| Online Access: | https://www.sciopen.com/article/10.26599/FSHW.2022.9250251 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850080007899054080 |
|---|---|
| author | Yao Wang Dongwei Wang Kai Wang Min Zhao Cizhou Li Yu Wang Xuwei Liu Lei Zhao Zhuoyan Hu |
| author_facet | Yao Wang Dongwei Wang Kai Wang Min Zhao Cizhou Li Yu Wang Xuwei Liu Lei Zhao Zhuoyan Hu |
| author_sort | Yao Wang |
| collection | DOAJ |
| description | Previous research reported litchi thaumatin-like protein (LcTLP) could lead to inflammation, which is a factor causing the adverse reactions after excessive intake of litchi. As a main amino acid in litchi pulp, γ-aminobutyric acid (GABA) was found with anti-inflammatory effect. Therefore, this study aimed to investigate the effects of GABA on LcTLP-induced inflammation through RAW264.7 macrophages and C57BL mice models. In vitro study showed GABA could effectively regulate the level of inflammatory cytokines (interleukin (IL)-1β, IL-6, IL-10, and prostaglandin E2) and Ca2+ in cells, and inhibit the phosphorylation of p65, I κB, p38, c-Jun N-terminal kinase (JNK) and extracellular signal-regulated kinase (ERK). These results indicate GABA alleviated inflammation through nuclear factor-κB and mitogen-activated protein kinase pathway signaling pathways. In vivo experiment was performed to verify the anti -inflammatory effect of GABA, and the results demonstrated that GABA reduced the inflammation and oxidative stress in the liver of LcTLP-treated mice, as it down-regulated the pro- inflammatory cytokines, malondialdehyde, aspartate transferase, and alanine transaminase. The relative expression of phosphorylated p38, JNK and ERK in mice liver with GABA treatment were reduced to 65%, 39% and 80% of the control group, respectively. Furthermore, GABA treatment enriched probiotic bacteria and decreased pathogenic bacteria in mice gut, which reveals GABA could effectively reduce the translocation of gut microbiota. |
| format | Article |
| id | doaj-art-167cc36a85e24413ac01afa6770c8fe3 |
| institution | DOAJ |
| issn | 2097-0765 2213-4530 |
| language | English |
| publishDate | 2024-09-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-167cc36a85e24413ac01afa6770c8fe32025-08-20T02:45:03ZengTsinghua University PressFood Science and Human Wellness2097-07652213-45302024-09-011353043305310.26599/FSHW.2022.9250251γ-Aminobutyric acid alleviates litchi thaumatin-like protein-induced inflammation and reduces gut microbial translocationYao Wang0Dongwei Wang1Kai Wang2Min Zhao3Cizhou Li4Yu Wang5Xuwei Liu6Lei Zhao7Zhuoyan Hu8Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou 510642, ChinaGuangzhou Wanglaoji Lychee Industry Development Co., Ltd., Guangzhou 510623, ChinaGuangzhou Wanglaoji Lychee Industry Development Co., Ltd., Guangzhou 510623, ChinaGuangzhou Wanglaoji Lychee Industry Development Co., Ltd., Guangzhou 510623, ChinaGuangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou 510642, ChinaPrevious research reported litchi thaumatin-like protein (LcTLP) could lead to inflammation, which is a factor causing the adverse reactions after excessive intake of litchi. As a main amino acid in litchi pulp, γ-aminobutyric acid (GABA) was found with anti-inflammatory effect. Therefore, this study aimed to investigate the effects of GABA on LcTLP-induced inflammation through RAW264.7 macrophages and C57BL mice models. In vitro study showed GABA could effectively regulate the level of inflammatory cytokines (interleukin (IL)-1β, IL-6, IL-10, and prostaglandin E2) and Ca2+ in cells, and inhibit the phosphorylation of p65, I κB, p38, c-Jun N-terminal kinase (JNK) and extracellular signal-regulated kinase (ERK). These results indicate GABA alleviated inflammation through nuclear factor-κB and mitogen-activated protein kinase pathway signaling pathways. In vivo experiment was performed to verify the anti -inflammatory effect of GABA, and the results demonstrated that GABA reduced the inflammation and oxidative stress in the liver of LcTLP-treated mice, as it down-regulated the pro- inflammatory cytokines, malondialdehyde, aspartate transferase, and alanine transaminase. The relative expression of phosphorylated p38, JNK and ERK in mice liver with GABA treatment were reduced to 65%, 39% and 80% of the control group, respectively. Furthermore, GABA treatment enriched probiotic bacteria and decreased pathogenic bacteria in mice gut, which reveals GABA could effectively reduce the translocation of gut microbiota.https://www.sciopen.com/article/10.26599/FSHW.2022.9250251anti-inflammationgut microbiotalitchi thaumatin-like proteinmitogen-activated protein kinase pathwayγ-aminobutyric acid |
| spellingShingle | Yao Wang Dongwei Wang Kai Wang Min Zhao Cizhou Li Yu Wang Xuwei Liu Lei Zhao Zhuoyan Hu γ-Aminobutyric acid alleviates litchi thaumatin-like protein-induced inflammation and reduces gut microbial translocation Food Science and Human Wellness anti-inflammation gut microbiota litchi thaumatin-like protein mitogen-activated protein kinase pathway γ-aminobutyric acid |
| title | γ-Aminobutyric acid alleviates litchi thaumatin-like protein-induced inflammation and reduces gut microbial translocation |
| title_full | γ-Aminobutyric acid alleviates litchi thaumatin-like protein-induced inflammation and reduces gut microbial translocation |
| title_fullStr | γ-Aminobutyric acid alleviates litchi thaumatin-like protein-induced inflammation and reduces gut microbial translocation |
| title_full_unstemmed | γ-Aminobutyric acid alleviates litchi thaumatin-like protein-induced inflammation and reduces gut microbial translocation |
| title_short | γ-Aminobutyric acid alleviates litchi thaumatin-like protein-induced inflammation and reduces gut microbial translocation |
| title_sort | γ aminobutyric acid alleviates litchi thaumatin like protein induced inflammation and reduces gut microbial translocation |
| topic | anti-inflammation gut microbiota litchi thaumatin-like protein mitogen-activated protein kinase pathway γ-aminobutyric acid |
| url | https://www.sciopen.com/article/10.26599/FSHW.2022.9250251 |
| work_keys_str_mv | AT yaowang gaminobutyricacidalleviateslitchithaumatinlikeproteininducedinflammationandreducesgutmicrobialtranslocation AT dongweiwang gaminobutyricacidalleviateslitchithaumatinlikeproteininducedinflammationandreducesgutmicrobialtranslocation AT kaiwang gaminobutyricacidalleviateslitchithaumatinlikeproteininducedinflammationandreducesgutmicrobialtranslocation AT minzhao gaminobutyricacidalleviateslitchithaumatinlikeproteininducedinflammationandreducesgutmicrobialtranslocation AT cizhouli gaminobutyricacidalleviateslitchithaumatinlikeproteininducedinflammationandreducesgutmicrobialtranslocation AT yuwang gaminobutyricacidalleviateslitchithaumatinlikeproteininducedinflammationandreducesgutmicrobialtranslocation AT xuweiliu gaminobutyricacidalleviateslitchithaumatinlikeproteininducedinflammationandreducesgutmicrobialtranslocation AT leizhao gaminobutyricacidalleviateslitchithaumatinlikeproteininducedinflammationandreducesgutmicrobialtranslocation AT zhuoyanhu gaminobutyricacidalleviateslitchithaumatinlikeproteininducedinflammationandreducesgutmicrobialtranslocation |