Immune-mediated regeneration of cell-free vascular grafts in an ovine model
Abstract We developed acellular tissue engineered vessels (ATEV) using small intestine submucosa (SIS) incorporating heparin and a novel protein named H2R5. ATEVs were implanted into the arterial circulation of an ovine animal model, demonstrating high primary patency rates over a period of three mo...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2025-03-01
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| Series: | npj Regenerative Medicine |
| Online Access: | https://doi.org/10.1038/s41536-025-00400-7 |
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| author | Bita Nasiri Arundhati Das Karthik Ramachandran Sai Harsha Bhamidipati Yulun Wu Shriramprasad Venkatesan Rudiyanto Gunawan Daniel D. Swartz Stelios T. Andreadis |
| author_facet | Bita Nasiri Arundhati Das Karthik Ramachandran Sai Harsha Bhamidipati Yulun Wu Shriramprasad Venkatesan Rudiyanto Gunawan Daniel D. Swartz Stelios T. Andreadis |
| author_sort | Bita Nasiri |
| collection | DOAJ |
| description | Abstract We developed acellular tissue engineered vessels (ATEV) using small intestine submucosa (SIS) incorporating heparin and a novel protein named H2R5. ATEVs were implanted into the arterial circulation of an ovine animal model, demonstrating high primary patency rates over a period of three months. Implanted grafts were infiltrated by host cells, the majority of which were monocytes/macrophages (MC/MΦ), as demonstrated by scRNA sequencing and immunostaining. They also developed functional endothelial and medial layers that deposited new extracellular matrix leading to matrix remodeling and acquisition of mechanical properties that were similar to those of native arteries. Notably, during this short implantation time, ATEVs turned into functional neo-arteries, as evidenced by the development of the vascular contractile function. Our findings underscore the potential of H2R5-functionalized ATEVs as promising candidates for tissue replacement grafts in a large pre-clinical animal model and highlight the contribution of macrophages in vascular regeneration. |
| format | Article |
| id | doaj-art-1677f3dd9a5b4f4590cebcfbdc494047 |
| institution | Kabale University |
| issn | 2057-3995 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | npj Regenerative Medicine |
| spelling | doaj-art-1677f3dd9a5b4f4590cebcfbdc4940472025-08-20T03:41:46ZengNature Portfolionpj Regenerative Medicine2057-39952025-03-0110111510.1038/s41536-025-00400-7Immune-mediated regeneration of cell-free vascular grafts in an ovine modelBita Nasiri0Arundhati Das1Karthik Ramachandran2Sai Harsha Bhamidipati3Yulun Wu4Shriramprasad Venkatesan5Rudiyanto Gunawan6Daniel D. Swartz7Stelios T. Andreadis8Department of Chemical and Biological Engineering, University at Buffalo, The State University of New YorkDepartment of Chemical and Biological Engineering, University at Buffalo, The State University of New YorkDepartment of Chemical and Biological Engineering, University at Buffalo, The State University of New YorkDepartment of Chemical and Biological Engineering, University at Buffalo, The State University of New YorkDepartment of Chemical and Biological Engineering, University at Buffalo, The State University of New YorkDepartment of Chemical and Biological Engineering, University at Buffalo, The State University of New YorkDepartment of Chemical and Biological Engineering, University at Buffalo, The State University of New YorkAngiograft LLCDepartment of Chemical and Biological Engineering, University at Buffalo, The State University of New YorkAbstract We developed acellular tissue engineered vessels (ATEV) using small intestine submucosa (SIS) incorporating heparin and a novel protein named H2R5. ATEVs were implanted into the arterial circulation of an ovine animal model, demonstrating high primary patency rates over a period of three months. Implanted grafts were infiltrated by host cells, the majority of which were monocytes/macrophages (MC/MΦ), as demonstrated by scRNA sequencing and immunostaining. They also developed functional endothelial and medial layers that deposited new extracellular matrix leading to matrix remodeling and acquisition of mechanical properties that were similar to those of native arteries. Notably, during this short implantation time, ATEVs turned into functional neo-arteries, as evidenced by the development of the vascular contractile function. Our findings underscore the potential of H2R5-functionalized ATEVs as promising candidates for tissue replacement grafts in a large pre-clinical animal model and highlight the contribution of macrophages in vascular regeneration.https://doi.org/10.1038/s41536-025-00400-7 |
| spellingShingle | Bita Nasiri Arundhati Das Karthik Ramachandran Sai Harsha Bhamidipati Yulun Wu Shriramprasad Venkatesan Rudiyanto Gunawan Daniel D. Swartz Stelios T. Andreadis Immune-mediated regeneration of cell-free vascular grafts in an ovine model npj Regenerative Medicine |
| title | Immune-mediated regeneration of cell-free vascular grafts in an ovine model |
| title_full | Immune-mediated regeneration of cell-free vascular grafts in an ovine model |
| title_fullStr | Immune-mediated regeneration of cell-free vascular grafts in an ovine model |
| title_full_unstemmed | Immune-mediated regeneration of cell-free vascular grafts in an ovine model |
| title_short | Immune-mediated regeneration of cell-free vascular grafts in an ovine model |
| title_sort | immune mediated regeneration of cell free vascular grafts in an ovine model |
| url | https://doi.org/10.1038/s41536-025-00400-7 |
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