Canopy management through crop forcing impacts the metabolism of phenolic compounds in grapevine canes

Despite extensive research on phenolic metabolism in grapevines, the effects of canopy management practices on the phenolic composition of woody canopy tissues remain insufficiently understood. This study investigates the impact of severe canopy management through crop forcing (CF) on the metabolic...

Full description

Saved in:
Bibliographic Details
Main Authors: António Teixeira, Inês L. Cabral, Henrique Noronha, Manon Ferrier, Marin-Pierre Gémin, Joana Valente, Fernando Alves, Susana M. P. Carvalho, Jorge Queiroz, Arnaud Lanoue, Hernâni Gerós
Format: Article
Language:English
Published: International Viticulture and Enology Society 2025-05-01
Series:OENO One
Subjects:
Online Access:https://oeno-one.eu/article/view/8245
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1850284262210666496
author António Teixeira
Inês L. Cabral
Henrique Noronha
Manon Ferrier
Marin-Pierre Gémin
Joana Valente
Fernando Alves
Susana M. P. Carvalho
Jorge Queiroz
Arnaud Lanoue
Hernâni Gerós
author_facet António Teixeira
Inês L. Cabral
Henrique Noronha
Manon Ferrier
Marin-Pierre Gémin
Joana Valente
Fernando Alves
Susana M. P. Carvalho
Jorge Queiroz
Arnaud Lanoue
Hernâni Gerós
author_sort António Teixeira
collection DOAJ
description Despite extensive research on phenolic metabolism in grapevines, the effects of canopy management practices on the phenolic composition of woody canopy tissues remain insufficiently understood. This study investigates the impact of severe canopy management through crop forcing (CF) on the metabolic profile of grapevine canes cv. ‘Touriga Nacional’, building on previous evidence that CF alters the vine physiology. A targeted UPLC-MS analysis was used to identify treatment effects on 54 metabolites, including phenolic acids, stilbenoids, flavonols, and flavan-3-ols. PCA and OPLS-DA showed that woody canes from CF vines have distinct metabolic profiles, with stilbenoids increasing consistently. Notably, there was an increase of E-resveratrol (7-fold), E-piceatannol (7-fold), and E-ε-viniferin (7-fold), compounds that are recognised to play crucial roles in boosting the plant’s defence mechanisms. On the other hand, flavan-3-ols like procyanidin B1 showed a consistent decrease in both vintages by up to 24 %. These findings suggest that CF promotes the accumulation of stilbenes and the reduction of flavonols and flavan-3-ols in grapevine canes.
format Article
id doaj-art-1642aff1ca2748c6a59d1a71b4d167ba
institution OA Journals
issn 2494-1271
language English
publishDate 2025-05-01
publisher International Viticulture and Enology Society
record_format Article
series OENO One
spelling doaj-art-1642aff1ca2748c6a59d1a71b4d167ba2025-08-20T01:47:37ZengInternational Viticulture and Enology SocietyOENO One2494-12712025-05-0159210.20870/oeno-one.2025.59.2.8245Canopy management through crop forcing impacts the metabolism of phenolic compounds in grapevine canesAntónio Teixeira0https://orcid.org/0000-0003-1942-7955Inês L. Cabral1Henrique Noronha2Manon Ferrier3Marin-Pierre Gémin4Joana Valente5Fernando Alves6Susana M. P. Carvalho7Jorge Queiroz8Arnaud Lanoue9Hernâni Gerós10Centre of Molecular and Environmental Biology (CBMA), Department of Biology, Campus de Gualtar, University do Minho, 4710-057 Braga, PortugalGreenUPorto—Research Centre on Sustainable Agrifood Production/Inov4Agro & DGAOT, Faculty of Sciences, Campus de Vairão, University of Porto, Rua da Agrária 747, 4485-646 Vairão, PortugalCentre of Molecular and Environmental Biology (CBMA), Department of Biology, Campus de Gualtar, University do Minho, 4710-057 Braga, PortugalEA2106 Biomolécules et Biotechnologies Végétales, Université de Tours, 37200 Tours, FranceEA2106 Biomolécules et Biotechnologies Végétales, Université de Tours, 37200 Tours, FranceSymington Family Estates, Vinhos SA, Travessa Barão de Forrester 86, 4431-901 Vila Nova de Gaia, PortugalSymington Family Estates, Vinhos SA, Travessa Barão de Forrester 86, 4431-901 Vila Nova de Gaia, PortugalGreenUPorto—Research Centre on Sustainable Agrifood Production/Inov4Agro & DGAOT, Faculty of Sciences, Campus de Vairão, University of Porto, Rua da Agrária 747, 4485-646 Vairão, PortugalGreenUPorto—Research Centre on Sustainable Agrifood Production/Inov4Agro & DGAOT, Faculty of Sciences, Campus de Vairão, University of Porto, Rua da Agrária 747, 4485-646 Vairão, PortugalEA2106 Biomolécules et Biotechnologies Végétales, Université de Tours, 37200 Tours, FranceCentre of Molecular and Environmental Biology (CBMA), Department of Biology, Campus de Gualtar, University do Minho, 4710-057 Braga, Portugal Despite extensive research on phenolic metabolism in grapevines, the effects of canopy management practices on the phenolic composition of woody canopy tissues remain insufficiently understood. This study investigates the impact of severe canopy management through crop forcing (CF) on the metabolic profile of grapevine canes cv. ‘Touriga Nacional’, building on previous evidence that CF alters the vine physiology. A targeted UPLC-MS analysis was used to identify treatment effects on 54 metabolites, including phenolic acids, stilbenoids, flavonols, and flavan-3-ols. PCA and OPLS-DA showed that woody canes from CF vines have distinct metabolic profiles, with stilbenoids increasing consistently. Notably, there was an increase of E-resveratrol (7-fold), E-piceatannol (7-fold), and E-ε-viniferin (7-fold), compounds that are recognised to play crucial roles in boosting the plant’s defence mechanisms. On the other hand, flavan-3-ols like procyanidin B1 showed a consistent decrease in both vintages by up to 24 %. These findings suggest that CF promotes the accumulation of stilbenes and the reduction of flavonols and flavan-3-ols in grapevine canes. https://oeno-one.eu/article/view/8245phenylpropanoid metabolismcanopy managementstilbenoidsflavan-3-olsmetabolic profile
spellingShingle António Teixeira
Inês L. Cabral
Henrique Noronha
Manon Ferrier
Marin-Pierre Gémin
Joana Valente
Fernando Alves
Susana M. P. Carvalho
Jorge Queiroz
Arnaud Lanoue
Hernâni Gerós
Canopy management through crop forcing impacts the metabolism of phenolic compounds in grapevine canes
OENO One
phenylpropanoid metabolism
canopy management
stilbenoids
flavan-3-ols
metabolic profile
title Canopy management through crop forcing impacts the metabolism of phenolic compounds in grapevine canes
title_full Canopy management through crop forcing impacts the metabolism of phenolic compounds in grapevine canes
title_fullStr Canopy management through crop forcing impacts the metabolism of phenolic compounds in grapevine canes
title_full_unstemmed Canopy management through crop forcing impacts the metabolism of phenolic compounds in grapevine canes
title_short Canopy management through crop forcing impacts the metabolism of phenolic compounds in grapevine canes
title_sort canopy management through crop forcing impacts the metabolism of phenolic compounds in grapevine canes
topic phenylpropanoid metabolism
canopy management
stilbenoids
flavan-3-ols
metabolic profile
url https://oeno-one.eu/article/view/8245
work_keys_str_mv AT antonioteixeira canopymanagementthroughcropforcingimpactsthemetabolismofphenoliccompoundsingrapevinecanes
AT ineslcabral canopymanagementthroughcropforcingimpactsthemetabolismofphenoliccompoundsingrapevinecanes
AT henriquenoronha canopymanagementthroughcropforcingimpactsthemetabolismofphenoliccompoundsingrapevinecanes
AT manonferrier canopymanagementthroughcropforcingimpactsthemetabolismofphenoliccompoundsingrapevinecanes
AT marinpierregemin canopymanagementthroughcropforcingimpactsthemetabolismofphenoliccompoundsingrapevinecanes
AT joanavalente canopymanagementthroughcropforcingimpactsthemetabolismofphenoliccompoundsingrapevinecanes
AT fernandoalves canopymanagementthroughcropforcingimpactsthemetabolismofphenoliccompoundsingrapevinecanes
AT susanampcarvalho canopymanagementthroughcropforcingimpactsthemetabolismofphenoliccompoundsingrapevinecanes
AT jorgequeiroz canopymanagementthroughcropforcingimpactsthemetabolismofphenoliccompoundsingrapevinecanes
AT arnaudlanoue canopymanagementthroughcropforcingimpactsthemetabolismofphenoliccompoundsingrapevinecanes
AT hernanigeros canopymanagementthroughcropforcingimpactsthemetabolismofphenoliccompoundsingrapevinecanes