Protocol for immunostaining of non-adherent cells and cellular structures using centrifugal filter devices

Summary: Immunostaining of non-adherent cells can be technically challenging due to the difficulty of efficiently exchanging solutions, a crucial process during immunostaining. Here, we present a protocol that utilizes centrifugal filter devices for immunostaining of non-adherent cells. We describe...

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Bibliographic Details
Main Authors: Momoko Miyazawa, Kentaro Kawai, Shohei Yamamoto, Daiju Kitagawa
Format: Article
Language:English
Published: Elsevier 2025-09-01
Series:STAR Protocols
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Online Access:http://www.sciencedirect.com/science/article/pii/S2666166725003405
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