Analysis of Recombinant Protein Expression by MALDI-TOF Mass Spectrometry of Bacterial Colonies
E. coli expressing soluble recombinant HIV antigens were analyzed directly by MALDI-TOF mass spectrometry (MS) from bacterial colonies picked from agar plates. An HIV envelope (ENV) antigen construct, penvA, was expressed in E. coli by transformation of the plasmid pPL/penvA-M. The plasmid was co-tr...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2000-05-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/00285st01 |
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