Unveiling the impact of 16S rRNA gene intergenomic variation on primer design and gut microbiome profiling

Unveiling the impact of 16S rRNA gene intergenomic variation on primer design and gut microbiome profiling

The 16S rRNA gene is crucial for bacterial identification, but primer biases and intergenomic variation can compromise its effectiveness, especially in complex ecosystems like the human gut microbiome. This study systematically evaluates 57 commonly used 16S rRNA primer sets through in silico PCR si...

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Bibliographic Details
Main Authors: Sirinthorn Sunthornthummas, Rujipat Wasitthankasem, Pimonwan Phokhaphan, Nirinya Sudtachat, Alisa Wilantho, Chumpol Ngamphiw, Wanwisa Chareanchim, Sissades Tongsima
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-05-01
Series:Frontiers in Microbiology
Subjects:
16S rRNA gene
gut microbiome profiling
primer design
intergenomic variation
microbial community
Online Access:https://www.frontiersin.org/articles/10.3389/fmicb.2025.1573920/full
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https://www.frontiersin.org/articles/10.3389/fmicb.2025.1573920/full

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