Safety assessment of a novel marine multi-stress-tolerant yeast Meyerozyma guilliermondii GXDK6 according to phenotype and whole genome-sequencing analysis
The application of microorganisms as probiotics is limited due to lack of safety evaluation. Here, a novel multi-stress-tolerant yeast Meyerozyma guilliermondii GXDK6 with aroma-producing properties was identified from marine mangrove microorganisms. Its safety and probiotic properties were assessed...
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| Format: | Article |
| Language: | English |
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Tsinghua University Press
2024-07-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | https://www.sciopen.com/article/10.26599/FSHW.2022.9250170 |
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| author | Xueyan Mo Mengcheng Zhou Yanmei Li Lili Yu Huashang Bai Peihong Shen Xing Zhou Haojun Zhu Huijie Sun Ru Bu Chengjian Jiang |
| author_facet | Xueyan Mo Mengcheng Zhou Yanmei Li Lili Yu Huashang Bai Peihong Shen Xing Zhou Haojun Zhu Huijie Sun Ru Bu Chengjian Jiang |
| author_sort | Xueyan Mo |
| collection | DOAJ |
| description | The application of microorganisms as probiotics is limited due to lack of safety evaluation. Here, a novel multi-stress-tolerant yeast Meyerozyma guilliermondii GXDK6 with aroma-producing properties was identified from marine mangrove microorganisms. Its safety and probiotic properties were assessed in accordance with phenotype and whole-genome sequencing analysis. Results showed that the genes and phenotypic expression of related virulence, antibiotic resistance and retroelement were rarely found. Hyphal morphogenesis genes (SIT4, HOG1, SPA2, ERK1, ICL1, CST20, HSP104, TPS1, and RHO1) and phospholipase secretion gene (VPS4) were annotated. True hyphae and phospholipase were absent. Only one retroelement (Tad1-65_BG) was found. Major biogenic amines (BAs) encoding genes were absent, except for spermidine synthase (JA9_002594), spermine synthase (JA9_004690), and tyrosine decarboxylase (inx). The production of single BAs and total BAs was far below the food-defined thresholds. GXDK6 had no resistance to common antifungal drugs. Virulence enzymes, such as gelatinase, DNase, hemolytic, lecithinase, and thrombin were absent. Acute toxicity test with mice demonstrated that GXDK6 is safe. GXDK6 has a good reproduction ability in the simulation gastrointestinal tract. GXDK6 also has a strong antioxidant ability, β-glucosidase, and inulinase activity. To sum up, GXDK6 is considered as a safe probiotic for human consumption and food fermentation. |
| format | Article |
| id | doaj-art-1479c64e4e4746c2aa2818dba0aa8da7 |
| institution | Kabale University |
| issn | 2097-0765 2213-4530 |
| language | English |
| publishDate | 2024-07-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-1479c64e4e4746c2aa2818dba0aa8da72025-01-10T06:56:48ZengTsinghua University PressFood Science and Human Wellness2097-07652213-45302024-07-011342048205910.26599/FSHW.2022.9250170Safety assessment of a novel marine multi-stress-tolerant yeast Meyerozyma guilliermondii GXDK6 according to phenotype and whole genome-sequencing analysisXueyan Mo0Mengcheng Zhou1Yanmei Li2Lili Yu3Huashang Bai4Peihong Shen5Xing Zhou6Haojun Zhu7Huijie Sun8Ru Bu9Chengjian Jiang10State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi Research Center for Microbial and Enzyme Engineering Technology, College of Life Science and Technology, Guangxi University, Nanning 530004, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi Research Center for Microbial and Enzyme Engineering Technology, College of Life Science and Technology, Guangxi University, Nanning 530004, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi Research Center for Microbial and Enzyme Engineering Technology, College of Life Science and Technology, Guangxi University, Nanning 530004, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi Research Center for Microbial and Enzyme Engineering Technology, College of Life Science and Technology, Guangxi University, Nanning 530004, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi Research Center for Microbial and Enzyme Engineering Technology, College of Life Science and Technology, Guangxi University, Nanning 530004, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi Research Center for Microbial and Enzyme Engineering Technology, College of Life Science and Technology, Guangxi University, Nanning 530004, ChinaNational Engineering Research Center for Non-Food Bioref inery, State Key Laboratory of Non-Food Biomass and Enzyme Technology, Guangxi Research Center for Biological Science and Technology, Guangxi Academy of Sciences, Nanning 530007, ChinaNational Engineering Research Center for Non-Food Bioref inery, State Key Laboratory of Non-Food Biomass and Enzyme Technology, Guangxi Research Center for Biological Science and Technology, Guangxi Academy of Sciences, Nanning 530007, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi Research Center for Microbial and Enzyme Engineering Technology, College of Life Science and Technology, Guangxi University, Nanning 530004, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi Research Center for Microbial and Enzyme Engineering Technology, College of Life Science and Technology, Guangxi University, Nanning 530004, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi Research Center for Microbial and Enzyme Engineering Technology, College of Life Science and Technology, Guangxi University, Nanning 530004, ChinaThe application of microorganisms as probiotics is limited due to lack of safety evaluation. Here, a novel multi-stress-tolerant yeast Meyerozyma guilliermondii GXDK6 with aroma-producing properties was identified from marine mangrove microorganisms. Its safety and probiotic properties were assessed in accordance with phenotype and whole-genome sequencing analysis. Results showed that the genes and phenotypic expression of related virulence, antibiotic resistance and retroelement were rarely found. Hyphal morphogenesis genes (SIT4, HOG1, SPA2, ERK1, ICL1, CST20, HSP104, TPS1, and RHO1) and phospholipase secretion gene (VPS4) were annotated. True hyphae and phospholipase were absent. Only one retroelement (Tad1-65_BG) was found. Major biogenic amines (BAs) encoding genes were absent, except for spermidine synthase (JA9_002594), spermine synthase (JA9_004690), and tyrosine decarboxylase (inx). The production of single BAs and total BAs was far below the food-defined thresholds. GXDK6 had no resistance to common antifungal drugs. Virulence enzymes, such as gelatinase, DNase, hemolytic, lecithinase, and thrombin were absent. Acute toxicity test with mice demonstrated that GXDK6 is safe. GXDK6 has a good reproduction ability in the simulation gastrointestinal tract. GXDK6 also has a strong antioxidant ability, β-glucosidase, and inulinase activity. To sum up, GXDK6 is considered as a safe probiotic for human consumption and food fermentation.https://www.sciopen.com/article/10.26599/FSHW.2022.9250170meyerozyma guilliermondiisafety assessmentprobioticsmarine mangrove microorganismswhole-genome sequencing analysis |
| spellingShingle | Xueyan Mo Mengcheng Zhou Yanmei Li Lili Yu Huashang Bai Peihong Shen Xing Zhou Haojun Zhu Huijie Sun Ru Bu Chengjian Jiang Safety assessment of a novel marine multi-stress-tolerant yeast Meyerozyma guilliermondii GXDK6 according to phenotype and whole genome-sequencing analysis Food Science and Human Wellness meyerozyma guilliermondii safety assessment probiotics marine mangrove microorganisms whole-genome sequencing analysis |
| title | Safety assessment of a novel marine multi-stress-tolerant yeast Meyerozyma guilliermondii GXDK6 according to phenotype and whole genome-sequencing analysis |
| title_full | Safety assessment of a novel marine multi-stress-tolerant yeast Meyerozyma guilliermondii GXDK6 according to phenotype and whole genome-sequencing analysis |
| title_fullStr | Safety assessment of a novel marine multi-stress-tolerant yeast Meyerozyma guilliermondii GXDK6 according to phenotype and whole genome-sequencing analysis |
| title_full_unstemmed | Safety assessment of a novel marine multi-stress-tolerant yeast Meyerozyma guilliermondii GXDK6 according to phenotype and whole genome-sequencing analysis |
| title_short | Safety assessment of a novel marine multi-stress-tolerant yeast Meyerozyma guilliermondii GXDK6 according to phenotype and whole genome-sequencing analysis |
| title_sort | safety assessment of a novel marine multi stress tolerant yeast meyerozyma guilliermondii gxdk6 according to phenotype and whole genome sequencing analysis |
| topic | meyerozyma guilliermondii safety assessment probiotics marine mangrove microorganisms whole-genome sequencing analysis |
| url | https://www.sciopen.com/article/10.26599/FSHW.2022.9250170 |
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