Pengaruh Pemberian Mesenchymal Stem Cells Wharton’s Jelly terhadap Ekspresi Gen PPAR-γ pada Tikus Alzheimer
The increased production of beta-amyloid marks Alzheimer's disease. PPAR-γ shows involvement in Alzheimer's disease. PPAR-γ Gene is suspected to reduce beta-amyloid plaques and reduce inflammation and oxidative stress in Alzheimer's disease. Mesenchymal Stem Cells Wharton's Jelly...
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| Format: | Article |
| Language: | English |
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Faculty of Medicine at Universitas Andalas
2023-10-01
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| Series: | Jurnal Kesehatan Andalas |
| Online Access: | http://jurnal.fk.unand.ac.id/index.php/jka/article/view/2142 |
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| author | Nabila Priscilla Putri Hirowati Ali Tofrizal Tofrizal Eryati Darwin Restu Susanti Hasmiwati Hasmiwati |
| author_facet | Nabila Priscilla Putri Hirowati Ali Tofrizal Tofrizal Eryati Darwin Restu Susanti Hasmiwati Hasmiwati |
| author_sort | Nabila Priscilla Putri |
| collection | DOAJ |
| description | The increased production of beta-amyloid marks Alzheimer's disease. PPAR-γ shows involvement in Alzheimer's disease. PPAR-γ Gene is suspected to reduce beta-amyloid plaques and reduce inflammation and oxidative stress in Alzheimer's disease. Mesenchymal Stem Cells Wharton's Jelly (MSC-WJ) is expected to increase the PPAR-γ Gene expression. Objective: To observed the PPAR-γ Gene expression in Alzheimer's Mice. Methods: The mice are induced using AlCl3 and were given MSC-WJ. This study was an experimental study with a post-test-only control group design on 18 samples of RNA from Alzheimer's mice that were divided into 3 groups. Negative control group (K-), positive control group (K+), and experimented group (P). The mean of PPAR-γ gene expression was obtained by comparing the PPAR-γ gene with the GAPDH gene. This study used semiquantitative methods using ImageJ. Data analysis was used with the Kruskal-Wallis test. It is significant if the value is <0,05. Results: The mean ratio of PPAR-γ gene expression that was obtained in K-, K+, and P were 0.12, 0.06, and 0.08, respectively. There were significant differences between each group, with a p-value of 0,023 (p<0,05). Conclusion: Giving MSC-WJ could increase the PPAR-γ gene expression. Further study using real-time PCR is highly needed to increase the MSC-WJ treatment in patients with Alzheimer's disease.
Keywords: Alzheimer disease, MSC-WJ, PPAR- γ |
| format | Article |
| id | doaj-art-1427c8e34c344b14aa3170404f8edf4e |
| institution | Kabale University |
| issn | 2301-7406 2615-1138 |
| language | English |
| publishDate | 2023-10-01 |
| publisher | Faculty of Medicine at Universitas Andalas |
| record_format | Article |
| series | Jurnal Kesehatan Andalas |
| spelling | doaj-art-1427c8e34c344b14aa3170404f8edf4e2025-02-03T02:11:28ZengFaculty of Medicine at Universitas AndalasJurnal Kesehatan Andalas2301-74062615-11382023-10-01122707510.25077/jka.v12i2.21421244Pengaruh Pemberian Mesenchymal Stem Cells Wharton’s Jelly terhadap Ekspresi Gen PPAR-γ pada Tikus AlzheimerNabila Priscilla Putri0Hirowati Ali1Tofrizal Tofrizal2Eryati Darwin3Restu Susanti4Hasmiwati Hasmiwati5Program Studi Kedokteran, Fakultas Kedokteran, Universitas AndalasDepartemen Biokimia, Fakultas Andalas, Univerisitas AndalasBagian Patologi Anatomi, Fakultas Kedokteran, Universitas AndalasDepartemen Histologi, Fakultas Kedokteran, Universitas AndalasDepartemen Neurologi, Fakultas Kedokteran, Universitas AndalasDepartemen Parasitologi, Fakultas Kedokteran, Universitas AndalasThe increased production of beta-amyloid marks Alzheimer's disease. PPAR-γ shows involvement in Alzheimer's disease. PPAR-γ Gene is suspected to reduce beta-amyloid plaques and reduce inflammation and oxidative stress in Alzheimer's disease. Mesenchymal Stem Cells Wharton's Jelly (MSC-WJ) is expected to increase the PPAR-γ Gene expression. Objective: To observed the PPAR-γ Gene expression in Alzheimer's Mice. Methods: The mice are induced using AlCl3 and were given MSC-WJ. This study was an experimental study with a post-test-only control group design on 18 samples of RNA from Alzheimer's mice that were divided into 3 groups. Negative control group (K-), positive control group (K+), and experimented group (P). The mean of PPAR-γ gene expression was obtained by comparing the PPAR-γ gene with the GAPDH gene. This study used semiquantitative methods using ImageJ. Data analysis was used with the Kruskal-Wallis test. It is significant if the value is <0,05. Results: The mean ratio of PPAR-γ gene expression that was obtained in K-, K+, and P were 0.12, 0.06, and 0.08, respectively. There were significant differences between each group, with a p-value of 0,023 (p<0,05). Conclusion: Giving MSC-WJ could increase the PPAR-γ gene expression. Further study using real-time PCR is highly needed to increase the MSC-WJ treatment in patients with Alzheimer's disease. Keywords: Alzheimer disease, MSC-WJ, PPAR- γhttp://jurnal.fk.unand.ac.id/index.php/jka/article/view/2142 |
| spellingShingle | Nabila Priscilla Putri Hirowati Ali Tofrizal Tofrizal Eryati Darwin Restu Susanti Hasmiwati Hasmiwati Pengaruh Pemberian Mesenchymal Stem Cells Wharton’s Jelly terhadap Ekspresi Gen PPAR-γ pada Tikus Alzheimer Jurnal Kesehatan Andalas |
| title | Pengaruh Pemberian Mesenchymal Stem Cells Wharton’s Jelly terhadap Ekspresi Gen PPAR-γ pada Tikus Alzheimer |
| title_full | Pengaruh Pemberian Mesenchymal Stem Cells Wharton’s Jelly terhadap Ekspresi Gen PPAR-γ pada Tikus Alzheimer |
| title_fullStr | Pengaruh Pemberian Mesenchymal Stem Cells Wharton’s Jelly terhadap Ekspresi Gen PPAR-γ pada Tikus Alzheimer |
| title_full_unstemmed | Pengaruh Pemberian Mesenchymal Stem Cells Wharton’s Jelly terhadap Ekspresi Gen PPAR-γ pada Tikus Alzheimer |
| title_short | Pengaruh Pemberian Mesenchymal Stem Cells Wharton’s Jelly terhadap Ekspresi Gen PPAR-γ pada Tikus Alzheimer |
| title_sort | pengaruh pemberian mesenchymal stem cells wharton s jelly terhadap ekspresi gen ppar γ pada tikus alzheimer |
| url | http://jurnal.fk.unand.ac.id/index.php/jka/article/view/2142 |
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