Protocol to quantify total intracellular magnesium in small samples using a fluorescent plate reader and fluorescent dye
Summary: Assessing total magnesium (Mg) becomes challenging when cell quantities are minimal. Here, we present a protocol to quantify total intracellular Mg in samples with as few as several thousand cells using a fluorescent plate reader and the fluorescent dye diaza-18-crown-6-hydroxyquinoline-5 (...
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| Main Authors: | Lorenzo Anconelli, Giovanna Farruggia, Marco Lombardo, Francesca Rossi, Martyna M. Rydzyk, Martina Rossi, Emil Malucelli, Concettina Cappadone, Jeanette A. Maier, Stefano Iotti |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-09-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166725003624 |
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