Protocol to quantify total intracellular magnesium in small samples using a fluorescent plate reader and fluorescent dye

Protocol to quantify total intracellular magnesium in small samples using a fluorescent plate reader and fluorescent dye

Summary: Assessing total magnesium (Mg) becomes challenging when cell quantities are minimal. Here, we present a protocol to quantify total intracellular Mg in samples with as few as several thousand cells using a fluorescent plate reader and the fluorescent dye diaza-18-crown-6-hydroxyquinoline-5 (...

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Bibliographic Details
Main Authors: Lorenzo Anconelli, Giovanna Farruggia, Marco Lombardo, Francesca Rossi, Martyna M. Rydzyk, Martina Rossi, Emil Malucelli, Concettina Cappadone, Jeanette A. Maier, Stefano Iotti
Format: Article
Language:English
Published: Elsevier 2025-09-01
Series:STAR Protocols
Subjects:
Cell Biology
Cancer
Clinical Protocol
Chemistry
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166725003624
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http://www.sciencedirect.com/science/article/pii/S2666166725003624

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