Considerations for antibody-based detection of NRF2 in human cells

Based on the knockdown and overexpression experiments, it is accepted that in Tris-glycine SDS-PAGE human NRF2 migrates above 100 kDa, depending on the percentage of the gel. In 8 % Tris-glycine gel, monoclonal anti-NRF2 antibodies detect NRF2 signal as three bands migrating between 100 and 130 kDa....

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Bibliographic Details
Main Authors: Alicja Dziadosz-Brzezińska, Sara Kusiński, Artur Piróg, Zuzanna Urban-Wójciuk, Monikaben Padariya, Umesh Kalathiya, Sachin Kote, Alicja Sznarkowska
Format: Article
Language:English
Published: Elsevier 2025-04-01
Series:Redox Biology
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Online Access:http://www.sciencedirect.com/science/article/pii/S221323172500062X
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