Butterfly Pea and Roselle Combination Extracts Reduce V-CAM, ICAM, and IL-6 Levels in High Fat Atherogenic Diet Rats
BACKGROUND: Atherosclerosis, driven by inflammation and oxidative stress, increases the risk of coronary heart disease (CHD). Flavonoids in butterfly pea and roselle are known for their potent antioxidant and anti-inflammatory properties. While their individual effects on cardiovascular health have...
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| Format: | Article |
| Language: | English |
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Secretariat of The Indonesian Biomedical Journal
2024-12-01
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| Series: | Indonesian Biomedical Journal |
| Online Access: | https://inabj.org/index.php/ibj/article/view/3361 |
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| author | Karnirius Harefa Ahmad Hafizullah Ritonga Riri Safitri Barita Aritonang Rahmad Gurusinga Irmayani Irmayani Sri Wulan |
| author_facet | Karnirius Harefa Ahmad Hafizullah Ritonga Riri Safitri Barita Aritonang Rahmad Gurusinga Irmayani Irmayani Sri Wulan |
| author_sort | Karnirius Harefa |
| collection | DOAJ |
| description | BACKGROUND: Atherosclerosis, driven by inflammation and oxidative stress, increases the risk of coronary heart disease (CHD). Flavonoids in butterfly pea and roselle are known for their potent antioxidant and anti-inflammatory properties. While their individual effects on cardiovascular health have been studied, no studies have explored the combined impact on atherosclerosis biomarkers, including vascular cell adhesion molecules (VCAM)-1, intercellular adhesion molecule (ICAM)-1, and interleukin (IL)-6. Therefore, this study was performed to evaluate the synergistic effects of butterfly pea and roselle combination extracts (BPRCE) on these biomarkers.
METHODS: A study with a post-test control group design using 36 male white rats was performed. The rats were randomly assigned to 6 groups; 1 group was fed with standard feed, while 5 groups were fed with a high-fat atherogenic diet (HFAD) to create atherosclerosis rat models. The HFAD rats were given either no treatment, sodium carboxymethylcellulose (Na-CMC), 300, 400, or 500 mg/kgBW BPRCE. Serum levels of VCAM-1, ICAM-1, and IL-6 of rats were measured using enzyme-linked immunosorbent assay (ELISA) methods.
RESULTS: Increasing doses of BPRCE resulted in a significant reduction in VCAM-1, ICAM-1, and IL-6 compared to the other groups. The group with the highest dose, 500 mg/kgBW BPRCE, showed the greatest reduction of VCAM-1 level (32.73±3.57 pg/mL), ICAM-1 level (5.68±1.17 ng/mL), and IL-6 levels (21.49±4.62 pg/mL).
CONCLUSION: Administration of BPRCE in atherosclerosis rats model reduces VCAM-1, ICAM-1, and IL-6 in a dose-dependent manner. This study showed that using BPRCE as a traditional remedy for preventing and treating CHD at an optimal dose of 500 mg/kgBW might be a potential future application in reducing atherosclerosis biomarkers.
KEYWORDS: VCAM-1, ICAM-1, IL-6, butterfly pea, rosella, atherosclerosis |
| format | Article |
| id | doaj-art-139f11dd7846424099fb44d46fc62526 |
| institution | Kabale University |
| issn | 2085-3297 2355-9179 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Secretariat of The Indonesian Biomedical Journal |
| record_format | Article |
| series | Indonesian Biomedical Journal |
| spelling | doaj-art-139f11dd7846424099fb44d46fc625262025-01-06T02:04:18ZengSecretariat of The Indonesian Biomedical JournalIndonesian Biomedical Journal2085-32972355-91792024-12-011665273310.18585/inabj.v16i6.3361606Butterfly Pea and Roselle Combination Extracts Reduce V-CAM, ICAM, and IL-6 Levels in High Fat Atherogenic Diet RatsKarnirius Harefa0Ahmad Hafizullah Ritonga1Riri Safitri2Barita Aritonang3Rahmad Gurusinga4Irmayani Irmayani5Sri Wulan6Institut Kesehatan Medistra Lubuk Pakam, Jl. Sudirman No.38, Deli Serdang 20512Institut Kesehatan Medistra Lubuk Pakam, Jl. Sudirman No.38, Deli Serdang 20512Institut Kesehatan Medistra Lubuk Pakam, Jl. Sudirman No.38, Deli Serdang 20512Institut Kesehatan Medistra Lubuk Pakam, Jl. Sudirman No.38, Deli Serdang 20512Institut Kesehatan Medistra Lubuk Pakam, Jl. Sudirman No.38, Deli Serdang 20512Institut Kesehatan Medistra Lubuk Pakam, Jl. Sudirman No.38, Deli Serdang 20512Institut Kesehatan Medistra Lubuk Pakam, Jl. Sudirman No.38, Deli Serdang 20512BACKGROUND: Atherosclerosis, driven by inflammation and oxidative stress, increases the risk of coronary heart disease (CHD). Flavonoids in butterfly pea and roselle are known for their potent antioxidant and anti-inflammatory properties. While their individual effects on cardiovascular health have been studied, no studies have explored the combined impact on atherosclerosis biomarkers, including vascular cell adhesion molecules (VCAM)-1, intercellular adhesion molecule (ICAM)-1, and interleukin (IL)-6. Therefore, this study was performed to evaluate the synergistic effects of butterfly pea and roselle combination extracts (BPRCE) on these biomarkers. METHODS: A study with a post-test control group design using 36 male white rats was performed. The rats were randomly assigned to 6 groups; 1 group was fed with standard feed, while 5 groups were fed with a high-fat atherogenic diet (HFAD) to create atherosclerosis rat models. The HFAD rats were given either no treatment, sodium carboxymethylcellulose (Na-CMC), 300, 400, or 500 mg/kgBW BPRCE. Serum levels of VCAM-1, ICAM-1, and IL-6 of rats were measured using enzyme-linked immunosorbent assay (ELISA) methods. RESULTS: Increasing doses of BPRCE resulted in a significant reduction in VCAM-1, ICAM-1, and IL-6 compared to the other groups. The group with the highest dose, 500 mg/kgBW BPRCE, showed the greatest reduction of VCAM-1 level (32.73±3.57 pg/mL), ICAM-1 level (5.68±1.17 ng/mL), and IL-6 levels (21.49±4.62 pg/mL). CONCLUSION: Administration of BPRCE in atherosclerosis rats model reduces VCAM-1, ICAM-1, and IL-6 in a dose-dependent manner. This study showed that using BPRCE as a traditional remedy for preventing and treating CHD at an optimal dose of 500 mg/kgBW might be a potential future application in reducing atherosclerosis biomarkers. KEYWORDS: VCAM-1, ICAM-1, IL-6, butterfly pea, rosella, atherosclerosishttps://inabj.org/index.php/ibj/article/view/3361 |
| spellingShingle | Karnirius Harefa Ahmad Hafizullah Ritonga Riri Safitri Barita Aritonang Rahmad Gurusinga Irmayani Irmayani Sri Wulan Butterfly Pea and Roselle Combination Extracts Reduce V-CAM, ICAM, and IL-6 Levels in High Fat Atherogenic Diet Rats Indonesian Biomedical Journal |
| title | Butterfly Pea and Roselle Combination Extracts Reduce V-CAM, ICAM, and IL-6 Levels in High Fat Atherogenic Diet Rats |
| title_full | Butterfly Pea and Roselle Combination Extracts Reduce V-CAM, ICAM, and IL-6 Levels in High Fat Atherogenic Diet Rats |
| title_fullStr | Butterfly Pea and Roselle Combination Extracts Reduce V-CAM, ICAM, and IL-6 Levels in High Fat Atherogenic Diet Rats |
| title_full_unstemmed | Butterfly Pea and Roselle Combination Extracts Reduce V-CAM, ICAM, and IL-6 Levels in High Fat Atherogenic Diet Rats |
| title_short | Butterfly Pea and Roselle Combination Extracts Reduce V-CAM, ICAM, and IL-6 Levels in High Fat Atherogenic Diet Rats |
| title_sort | butterfly pea and roselle combination extracts reduce v cam icam and il 6 levels in high fat atherogenic diet rats |
| url | https://inabj.org/index.php/ibj/article/view/3361 |
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