Gene Editing in Pluripotent Stem Cells and Their Derived Organoids
With the rapid rise in gene-editing technology, pluripotent stem cells (PSCs) and their derived organoids have increasingly broader and practical applications in regenerative medicine. Gene-editing technologies, from large-scale nucleic acid endonucleases to CRISPR, have ignited a global research an...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2021-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2021/8130828 |
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| _version_ | 1832562787696508928 |
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| author | Hang Zhou Yun Wang Li-Ping Liu Yu-Mei Li Yun-Wen Zheng |
| author_facet | Hang Zhou Yun Wang Li-Ping Liu Yu-Mei Li Yun-Wen Zheng |
| author_sort | Hang Zhou |
| collection | DOAJ |
| description | With the rapid rise in gene-editing technology, pluripotent stem cells (PSCs) and their derived organoids have increasingly broader and practical applications in regenerative medicine. Gene-editing technologies, from large-scale nucleic acid endonucleases to CRISPR, have ignited a global research and development boom with significant implications in regenerative medicine. The development of regenerative medicine technologies, regardless of whether it is PSCs or gene editing, is consistently met with controversy. Are the tools for rewriting the code of life a boon to humanity or a Pandora’s box? These technologies raise concerns regarding ethical issues, unexpected mutations, viral infection, etc. These concerns remain even as new treatments emerge. However, the potential negatives cannot obscure the virtues of PSC gene editing, which have, and will continue to, benefit mankind at an unprecedented rate. Here, we briefly introduce current gene-editing technology and its application in PSCs and their derived organoids, while addressing ethical concerns and safety risks and discussing the latest progress in PSC gene editing. Gene editing in PSCs creates visualized in vitro models, providing opportunities for examining mechanisms of known and unknown mutations and offering new possibilities for the treatment of cancer, genetic diseases, and other serious or refractory disorders. From model construction to treatment exploration, the important role of PSCs combined with gene editing in basic and clinical medicine studies is illustrated. The applications, characteristics, and existing challenges are summarized in combination with our lab experiences in this field in an effort to help gene-editing technology better serve humans in a regulated manner. Current preclinical and clinical trials have demonstrated initial safety and efficacy of PSC gene editing; however, for better application in clinical settings, additional investigation is warranted. |
| format | Article |
| id | doaj-art-1362a29771304b6a96c7b91b16f23f11 |
| institution | Kabale University |
| issn | 1687-9678 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-1362a29771304b6a96c7b91b16f23f112025-02-03T01:21:45ZengWileyStem Cells International1687-96782021-01-01202110.1155/2021/8130828Gene Editing in Pluripotent Stem Cells and Their Derived OrganoidsHang Zhou0Yun Wang1Li-Ping Liu2Yu-Mei Li3Yun-Wen Zheng4Institute of Regenerative MedicineInstitute of Regenerative MedicineInstitute of Regenerative MedicineInstitute of Regenerative MedicineInstitute of Regenerative MedicineWith the rapid rise in gene-editing technology, pluripotent stem cells (PSCs) and their derived organoids have increasingly broader and practical applications in regenerative medicine. Gene-editing technologies, from large-scale nucleic acid endonucleases to CRISPR, have ignited a global research and development boom with significant implications in regenerative medicine. The development of regenerative medicine technologies, regardless of whether it is PSCs or gene editing, is consistently met with controversy. Are the tools for rewriting the code of life a boon to humanity or a Pandora’s box? These technologies raise concerns regarding ethical issues, unexpected mutations, viral infection, etc. These concerns remain even as new treatments emerge. However, the potential negatives cannot obscure the virtues of PSC gene editing, which have, and will continue to, benefit mankind at an unprecedented rate. Here, we briefly introduce current gene-editing technology and its application in PSCs and their derived organoids, while addressing ethical concerns and safety risks and discussing the latest progress in PSC gene editing. Gene editing in PSCs creates visualized in vitro models, providing opportunities for examining mechanisms of known and unknown mutations and offering new possibilities for the treatment of cancer, genetic diseases, and other serious or refractory disorders. From model construction to treatment exploration, the important role of PSCs combined with gene editing in basic and clinical medicine studies is illustrated. The applications, characteristics, and existing challenges are summarized in combination with our lab experiences in this field in an effort to help gene-editing technology better serve humans in a regulated manner. Current preclinical and clinical trials have demonstrated initial safety and efficacy of PSC gene editing; however, for better application in clinical settings, additional investigation is warranted.http://dx.doi.org/10.1155/2021/8130828 |
| spellingShingle | Hang Zhou Yun Wang Li-Ping Liu Yu-Mei Li Yun-Wen Zheng Gene Editing in Pluripotent Stem Cells and Their Derived Organoids Stem Cells International |
| title | Gene Editing in Pluripotent Stem Cells and Their Derived Organoids |
| title_full | Gene Editing in Pluripotent Stem Cells and Their Derived Organoids |
| title_fullStr | Gene Editing in Pluripotent Stem Cells and Their Derived Organoids |
| title_full_unstemmed | Gene Editing in Pluripotent Stem Cells and Their Derived Organoids |
| title_short | Gene Editing in Pluripotent Stem Cells and Their Derived Organoids |
| title_sort | gene editing in pluripotent stem cells and their derived organoids |
| url | http://dx.doi.org/10.1155/2021/8130828 |
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