Effect of air temperature, pH and nutrition on growth and sporulation of Ascochyta pisi

Isolates of Ascochyta pisi from diseased pea (Pisum sativum) varied greatly in growth rate, sporulation, mycelial appearance, physiology and pathogenicity. Best sporulation of A. pisi occurred on V-8 juice at 24oC, whereas best mycelial growth was on Czapeck medium and corn cockle (Agrostemma githag...

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Main Authors: R. L. SUSURI, D. J. HAGEDORN, R. E. RAND
Format: Article
Language:English
Published: University of Ljubljana Press (Založba Univerze v Ljubljani) 1999-03-01
Series:Acta Agriculturae Slovenica
Online Access:https://journals.uni-lj.si/aas/article/view/15965
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author R. L. SUSURI
D. J. HAGEDORN
R. E. RAND
author_facet R. L. SUSURI
D. J. HAGEDORN
R. E. RAND
author_sort R. L. SUSURI
collection DOAJ
description Isolates of Ascochyta pisi from diseased pea (Pisum sativum) varied greatly in growth rate, sporulation, mycelial appearance, physiology and pathogenicity. Best sporulation of A. pisi occurred on V-8 juice at 24oC, whereas best mycelial growth was on Czapeck medium and corn cockle (Agrostemma githago) seed meal agar. In pure culture on PDA A. pisi grew between 16 and 28oC with optimum at 24oC. Both isolates (A and P) grew well on medium with pH 3.8 to 7.3. As carbon sources maltose, dextrin and cellobiose supported best growth of isolates. Peptone, ammonium nitrate, ammonium sulphate and urea, as sources of nitrogen, promoted the best growth. The maximum vegetative growth is obtained with proline and casein hydroliysate for isolate A, whereas proline, aspartic acid and leucine as amino nitrogen sources for isolate P.
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spelling doaj-art-11f8a44eebbc4ca9bbb2598a71975a8e2025-08-20T03:02:25ZengUniversity of Ljubljana Press (Založba Univerze v Ljubljani)Acta Agriculturae Slovenica1854-19411999-03-01731717910.14720/aas.1999.73.1.1596522358Effect of air temperature, pH and nutrition on growth and sporulation of Ascochyta pisiR. L. SUSURID. J. HAGEDORNR. E. RANDIsolates of Ascochyta pisi from diseased pea (Pisum sativum) varied greatly in growth rate, sporulation, mycelial appearance, physiology and pathogenicity. Best sporulation of A. pisi occurred on V-8 juice at 24oC, whereas best mycelial growth was on Czapeck medium and corn cockle (Agrostemma githago) seed meal agar. In pure culture on PDA A. pisi grew between 16 and 28oC with optimum at 24oC. Both isolates (A and P) grew well on medium with pH 3.8 to 7.3. As carbon sources maltose, dextrin and cellobiose supported best growth of isolates. Peptone, ammonium nitrate, ammonium sulphate and urea, as sources of nitrogen, promoted the best growth. The maximum vegetative growth is obtained with proline and casein hydroliysate for isolate A, whereas proline, aspartic acid and leucine as amino nitrogen sources for isolate P.https://journals.uni-lj.si/aas/article/view/15965
spellingShingle R. L. SUSURI
D. J. HAGEDORN
R. E. RAND
Effect of air temperature, pH and nutrition on growth and sporulation of Ascochyta pisi
Acta Agriculturae Slovenica
title Effect of air temperature, pH and nutrition on growth and sporulation of Ascochyta pisi
title_full Effect of air temperature, pH and nutrition on growth and sporulation of Ascochyta pisi
title_fullStr Effect of air temperature, pH and nutrition on growth and sporulation of Ascochyta pisi
title_full_unstemmed Effect of air temperature, pH and nutrition on growth and sporulation of Ascochyta pisi
title_short Effect of air temperature, pH and nutrition on growth and sporulation of Ascochyta pisi
title_sort effect of air temperature ph and nutrition on growth and sporulation of ascochyta pisi
url https://journals.uni-lj.si/aas/article/view/15965
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