Multifaceted Approaches in Epithelial Cell Adhesion Molecule-Mediated Circulating Tumor Cell Isolation
Circulating tumor cells (CTCs) are pivotal in cancer metastasis and serve as valuable biomarkers for diagnosis, prognosis, and treatment monitoring. Traditional CTC capture methods predominantly utilize the epithelial cell adhesion molecule (EpCAM) as a marker for isolation. However, the heterogenei...
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MDPI AG
2025-02-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/30/5/976 |
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| author | Dora Szerenyi Gabor Jarvas Andras Guttman |
| author_facet | Dora Szerenyi Gabor Jarvas Andras Guttman |
| author_sort | Dora Szerenyi |
| collection | DOAJ |
| description | Circulating tumor cells (CTCs) are pivotal in cancer metastasis and serve as valuable biomarkers for diagnosis, prognosis, and treatment monitoring. Traditional CTC capture methods predominantly utilize the epithelial cell adhesion molecule (EpCAM) as a marker for isolation. However, the heterogeneity of these circulating cells and the epithelial-to-mesenchymal transition process (wherein epithelial cells acquire mesenchymal characteristics) limit the efficacy of EpCAM-based capture techniques. In this paper, we critically review the role of the EpCAM in CTC capture, explore the impact of epithelial-to-mesenchymal transition on EpCAM expression, and discuss alternative biomarkers and strategies to enhance CTC isolation. By evaluating the limitations of EpCAM-mediated capture and the challenges posed by epithelial-to-mesenchymal transition, we aim to provide insights into the development of more comprehensive liquid biopsy approaches for cancer management. |
| format | Article |
| id | doaj-art-118c449d45ea4000bce3f22db6fe64c0 |
| institution | DOAJ |
| issn | 1420-3049 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj-art-118c449d45ea4000bce3f22db6fe64c02025-08-20T02:53:21ZengMDPI AGMolecules1420-30492025-02-0130597610.3390/molecules30050976Multifaceted Approaches in Epithelial Cell Adhesion Molecule-Mediated Circulating Tumor Cell IsolationDora Szerenyi0Gabor Jarvas1Andras Guttman2Research Institute of Biomolecular and Chemical Engineering, Faculty of Engineering, University of Pannonia, 8200 Veszprem, HungaryResearch Institute of Biomolecular and Chemical Engineering, Faculty of Engineering, University of Pannonia, 8200 Veszprem, HungaryResearch Institute of Biomolecular and Chemical Engineering, Faculty of Engineering, University of Pannonia, 8200 Veszprem, HungaryCirculating tumor cells (CTCs) are pivotal in cancer metastasis and serve as valuable biomarkers for diagnosis, prognosis, and treatment monitoring. Traditional CTC capture methods predominantly utilize the epithelial cell adhesion molecule (EpCAM) as a marker for isolation. However, the heterogeneity of these circulating cells and the epithelial-to-mesenchymal transition process (wherein epithelial cells acquire mesenchymal characteristics) limit the efficacy of EpCAM-based capture techniques. In this paper, we critically review the role of the EpCAM in CTC capture, explore the impact of epithelial-to-mesenchymal transition on EpCAM expression, and discuss alternative biomarkers and strategies to enhance CTC isolation. By evaluating the limitations of EpCAM-mediated capture and the challenges posed by epithelial-to-mesenchymal transition, we aim to provide insights into the development of more comprehensive liquid biopsy approaches for cancer management.https://www.mdpi.com/1420-3049/30/5/976liquid biopsyCTCsurface markersEMTimmuno-affinitylabel-free isolation |
| spellingShingle | Dora Szerenyi Gabor Jarvas Andras Guttman Multifaceted Approaches in Epithelial Cell Adhesion Molecule-Mediated Circulating Tumor Cell Isolation Molecules liquid biopsy CTC surface markers EMT immuno-affinity label-free isolation |
| title | Multifaceted Approaches in Epithelial Cell Adhesion Molecule-Mediated Circulating Tumor Cell Isolation |
| title_full | Multifaceted Approaches in Epithelial Cell Adhesion Molecule-Mediated Circulating Tumor Cell Isolation |
| title_fullStr | Multifaceted Approaches in Epithelial Cell Adhesion Molecule-Mediated Circulating Tumor Cell Isolation |
| title_full_unstemmed | Multifaceted Approaches in Epithelial Cell Adhesion Molecule-Mediated Circulating Tumor Cell Isolation |
| title_short | Multifaceted Approaches in Epithelial Cell Adhesion Molecule-Mediated Circulating Tumor Cell Isolation |
| title_sort | multifaceted approaches in epithelial cell adhesion molecule mediated circulating tumor cell isolation |
| topic | liquid biopsy CTC surface markers EMT immuno-affinity label-free isolation |
| url | https://www.mdpi.com/1420-3049/30/5/976 |
| work_keys_str_mv | AT doraszerenyi multifacetedapproachesinepithelialcelladhesionmoleculemediatedcirculatingtumorcellisolation AT gaborjarvas multifacetedapproachesinepithelialcelladhesionmoleculemediatedcirculatingtumorcellisolation AT andrasguttman multifacetedapproachesinepithelialcelladhesionmoleculemediatedcirculatingtumorcellisolation |