Mutation Analysis of Synthetic DNA Barcodes in a Fission Yeast Gene Deletion Library by Sanger Sequencing

Mutation Analysis of Synthetic DNA Barcodes in a Fission Yeast Gene Deletion Library by Sanger Sequencing

Incorporation of unique barcodes into fission yeast gene deletion collections has enabled the identification of gene functions by growth fitness analysis. For fine tuning, it is important to examine barcode sequences, because mutations arise during strain construction. Out of 8,708 barcodes (4,354 s...

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Bibliographic Details
Main Authors: Minho Lee, Shin-Jung Choi, Sangjo Han, Miyoung Nam, Dongsup Kim, Dong-Uk Kim, Kwang-Lae Hoe
Format: Article
Language:English
Published: BioMed Central 2018-06-01
Series:Genomics & Informatics
Subjects:
barcode
fission yeast
gene deletion
growth fitness
mutation
Online Access:http://genominfo.org/upload/pdf/gi-2018-16-2-22.pdf
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http://genominfo.org/upload/pdf/gi-2018-16-2-22.pdf

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