Isothermal Amplification and Multimerization of DNA by Bst DNA Polymerase

We have demonstrated the isothermal in vitro amplification and multimerization of several different linear DNA targets using only two primers and the strongly strand-displacing exonuclease-negative Bst DNA polymerase. This reaction has been termed linear target isothermal multimerization and amplifi...

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Bibliographic Details
Main Authors: G.J. Hafner, I.C. Yang, L.C. Wolter, M.R. Stafford, P.M. Giffard
Format: Article
Language:English
Published: Taylor & Francis Group 2001-04-01
Series:BioTechniques
Online Access:https://www.future-science.com/doi/10.2144/01304rr03
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