Evaluation of Safety, Immunogenicity and Cross-Reactive Immunity of OVX836, a Nucleoprotein-Based Universal Influenza Vaccine, in Older Adults

<b>Background/Objectives:</b> In a Phase 2a, double-blind, placebo-controlled study including healthy participants aged 18–55 years, OVX836, a nucleoprotein (NP)-based candidate vaccine, previously showed a good safety profile, a robust immune response (both humoral and cellular) and a p...

Full description

Saved in:
Bibliographic Details
Main Authors: Bart Jacobs, Isabel Leroux-Roels, Jacques Bruhwyler, Nicola Groth, Gwenn Waerlop, Yorick Janssens, Jessika Tourneur, Fien De Boever, Azhar Alhatemi, Philippe Moris, Alexandre Le Vert, Geert Leroux-Roels, Florence Nicolas
Format: Article
Language:English
Published: MDPI AG 2024-12-01
Series:Vaccines
Subjects:
Online Access:https://www.mdpi.com/2076-393X/12/12/1391
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1850238281690644480
author Bart Jacobs
Isabel Leroux-Roels
Jacques Bruhwyler
Nicola Groth
Gwenn Waerlop
Yorick Janssens
Jessika Tourneur
Fien De Boever
Azhar Alhatemi
Philippe Moris
Alexandre Le Vert
Geert Leroux-Roels
Florence Nicolas
author_facet Bart Jacobs
Isabel Leroux-Roels
Jacques Bruhwyler
Nicola Groth
Gwenn Waerlop
Yorick Janssens
Jessika Tourneur
Fien De Boever
Azhar Alhatemi
Philippe Moris
Alexandre Le Vert
Geert Leroux-Roels
Florence Nicolas
author_sort Bart Jacobs
collection DOAJ
description <b>Background/Objectives:</b> In a Phase 2a, double-blind, placebo-controlled study including healthy participants aged 18–55 years, OVX836, a nucleoprotein (NP)-based candidate vaccine, previously showed a good safety profile, a robust immune response (both humoral and cellular) and a preliminary signal of protection (VE = 84%) against PCR-confirmed symptomatic influenza after a single intramuscular dose of 180 µg, 300 µg or 480 µg. <b>Methods</b>: Using the same methodology, we confirmed the good safety and strong immunogenicity of OVX836 at the same doses in older adults (≥65 years), a key target population for influenza vaccination. <b>Results</b>: Significant humoral (anti-NP IgG) and cellular (interferon gamma (IFNγ) spot-forming cells per million peripheral blood mononuclear cells and specific CD4<sup>+</sup> IFNγ<sup>+</sup> T-cells) immune responses were observed at the three dose levels, without clear dose–response relationship. T-cell responses were shown to be highly cross-reactive against various influenza A strains, both seasonal and highly pathogenic avian strains. We also evaluated the effect of sex (stronger immune response in females) and age (stronger immune response in young adults) on the immune response to OVX836 after adjustment based on the pre-vaccination immune status. <b>Conclusions</b>: The results obtained with OVX836 lay the groundwork for a future placebo-controlled, field proof of concept efficacy Phase 2b trial.
format Article
id doaj-art-0f7330faeb524d27b11dd357f6367a12
institution OA Journals
issn 2076-393X
language English
publishDate 2024-12-01
publisher MDPI AG
record_format Article
series Vaccines
spelling doaj-art-0f7330faeb524d27b11dd357f6367a122025-08-20T02:01:29ZengMDPI AGVaccines2076-393X2024-12-011212139110.3390/vaccines12121391Evaluation of Safety, Immunogenicity and Cross-Reactive Immunity of OVX836, a Nucleoprotein-Based Universal Influenza Vaccine, in Older AdultsBart Jacobs0Isabel Leroux-Roels1Jacques Bruhwyler2Nicola Groth3Gwenn Waerlop4Yorick Janssens5Jessika Tourneur6Fien De Boever7Azhar Alhatemi8Philippe Moris9Alexandre Le Vert10Geert Leroux-Roels11Florence Nicolas12Center for Vaccinology (CEVAC), 10 Corneel Heymanslaan, 9000 Ghent, BelgiumCenter for Vaccinology (CEVAC), 10 Corneel Heymanslaan, 9000 Ghent, BelgiumOsivax, 70 Rue Saint-Jean-de-Dieu, 69007 Lyon, FranceOsivax, 70 Rue Saint-Jean-de-Dieu, 69007 Lyon, FranceCenter for Vaccinology (CEVAC), 10 Corneel Heymanslaan, 9000 Ghent, BelgiumCenter for Vaccinology (CEVAC), 10 Corneel Heymanslaan, 9000 Ghent, BelgiumOsivax, 70 Rue Saint-Jean-de-Dieu, 69007 Lyon, FranceCenter for Vaccinology (CEVAC), 10 Corneel Heymanslaan, 9000 Ghent, BelgiumCenter for Vaccinology (CEVAC), 10 Corneel Heymanslaan, 9000 Ghent, BelgiumOsivax, 70 Rue Saint-Jean-de-Dieu, 69007 Lyon, FranceOsivax, 70 Rue Saint-Jean-de-Dieu, 69007 Lyon, FranceCenter for Vaccinology (CEVAC), 10 Corneel Heymanslaan, 9000 Ghent, BelgiumOsivax, 70 Rue Saint-Jean-de-Dieu, 69007 Lyon, France<b>Background/Objectives:</b> In a Phase 2a, double-blind, placebo-controlled study including healthy participants aged 18–55 years, OVX836, a nucleoprotein (NP)-based candidate vaccine, previously showed a good safety profile, a robust immune response (both humoral and cellular) and a preliminary signal of protection (VE = 84%) against PCR-confirmed symptomatic influenza after a single intramuscular dose of 180 µg, 300 µg or 480 µg. <b>Methods</b>: Using the same methodology, we confirmed the good safety and strong immunogenicity of OVX836 at the same doses in older adults (≥65 years), a key target population for influenza vaccination. <b>Results</b>: Significant humoral (anti-NP IgG) and cellular (interferon gamma (IFNγ) spot-forming cells per million peripheral blood mononuclear cells and specific CD4<sup>+</sup> IFNγ<sup>+</sup> T-cells) immune responses were observed at the three dose levels, without clear dose–response relationship. T-cell responses were shown to be highly cross-reactive against various influenza A strains, both seasonal and highly pathogenic avian strains. We also evaluated the effect of sex (stronger immune response in females) and age (stronger immune response in young adults) on the immune response to OVX836 after adjustment based on the pre-vaccination immune status. <b>Conclusions</b>: The results obtained with OVX836 lay the groundwork for a future placebo-controlled, field proof of concept efficacy Phase 2b trial.https://www.mdpi.com/2076-393X/12/12/1391influenzauniversal vaccinePhase 2ahealthy participantsolder adultscross-reactivity
spellingShingle Bart Jacobs
Isabel Leroux-Roels
Jacques Bruhwyler
Nicola Groth
Gwenn Waerlop
Yorick Janssens
Jessika Tourneur
Fien De Boever
Azhar Alhatemi
Philippe Moris
Alexandre Le Vert
Geert Leroux-Roels
Florence Nicolas
Evaluation of Safety, Immunogenicity and Cross-Reactive Immunity of OVX836, a Nucleoprotein-Based Universal Influenza Vaccine, in Older Adults
Vaccines
influenza
universal vaccine
Phase 2a
healthy participants
older adults
cross-reactivity
title Evaluation of Safety, Immunogenicity and Cross-Reactive Immunity of OVX836, a Nucleoprotein-Based Universal Influenza Vaccine, in Older Adults
title_full Evaluation of Safety, Immunogenicity and Cross-Reactive Immunity of OVX836, a Nucleoprotein-Based Universal Influenza Vaccine, in Older Adults
title_fullStr Evaluation of Safety, Immunogenicity and Cross-Reactive Immunity of OVX836, a Nucleoprotein-Based Universal Influenza Vaccine, in Older Adults
title_full_unstemmed Evaluation of Safety, Immunogenicity and Cross-Reactive Immunity of OVX836, a Nucleoprotein-Based Universal Influenza Vaccine, in Older Adults
title_short Evaluation of Safety, Immunogenicity and Cross-Reactive Immunity of OVX836, a Nucleoprotein-Based Universal Influenza Vaccine, in Older Adults
title_sort evaluation of safety immunogenicity and cross reactive immunity of ovx836 a nucleoprotein based universal influenza vaccine in older adults
topic influenza
universal vaccine
Phase 2a
healthy participants
older adults
cross-reactivity
url https://www.mdpi.com/2076-393X/12/12/1391
work_keys_str_mv AT bartjacobs evaluationofsafetyimmunogenicityandcrossreactiveimmunityofovx836anucleoproteinbaseduniversalinfluenzavaccineinolderadults
AT isabellerouxroels evaluationofsafetyimmunogenicityandcrossreactiveimmunityofovx836anucleoproteinbaseduniversalinfluenzavaccineinolderadults
AT jacquesbruhwyler evaluationofsafetyimmunogenicityandcrossreactiveimmunityofovx836anucleoproteinbaseduniversalinfluenzavaccineinolderadults
AT nicolagroth evaluationofsafetyimmunogenicityandcrossreactiveimmunityofovx836anucleoproteinbaseduniversalinfluenzavaccineinolderadults
AT gwennwaerlop evaluationofsafetyimmunogenicityandcrossreactiveimmunityofovx836anucleoproteinbaseduniversalinfluenzavaccineinolderadults
AT yorickjanssens evaluationofsafetyimmunogenicityandcrossreactiveimmunityofovx836anucleoproteinbaseduniversalinfluenzavaccineinolderadults
AT jessikatourneur evaluationofsafetyimmunogenicityandcrossreactiveimmunityofovx836anucleoproteinbaseduniversalinfluenzavaccineinolderadults
AT fiendeboever evaluationofsafetyimmunogenicityandcrossreactiveimmunityofovx836anucleoproteinbaseduniversalinfluenzavaccineinolderadults
AT azharalhatemi evaluationofsafetyimmunogenicityandcrossreactiveimmunityofovx836anucleoproteinbaseduniversalinfluenzavaccineinolderadults
AT philippemoris evaluationofsafetyimmunogenicityandcrossreactiveimmunityofovx836anucleoproteinbaseduniversalinfluenzavaccineinolderadults
AT alexandrelevert evaluationofsafetyimmunogenicityandcrossreactiveimmunityofovx836anucleoproteinbaseduniversalinfluenzavaccineinolderadults
AT geertlerouxroels evaluationofsafetyimmunogenicityandcrossreactiveimmunityofovx836anucleoproteinbaseduniversalinfluenzavaccineinolderadults
AT florencenicolas evaluationofsafetyimmunogenicityandcrossreactiveimmunityofovx836anucleoproteinbaseduniversalinfluenzavaccineinolderadults