TRIM47 promotes head and neck squamous cell carcinoma malignant progression by degrading XAF1 through ubiquitination
Summary: Tripartite motif-containing 47 (TRIM47) is a member of the TRIM family, which has E3 ligase activity and has been demonstrated to be involved in tumor development. In this work, we found that TRIM47 is highly expressed in head and neck squamous cell carcinomas (HNSCC) tissues. TRIM47 overex...
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Elsevier
2025-01-01
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| Series: | iScience |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004224028177 |
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| author | Changyun Yu Chen Zhang Qianqian Zhang Cai Zhang Jingjie Han Jinying Li |
| author_facet | Changyun Yu Chen Zhang Qianqian Zhang Cai Zhang Jingjie Han Jinying Li |
| author_sort | Changyun Yu |
| collection | DOAJ |
| description | Summary: Tripartite motif-containing 47 (TRIM47) is a member of the TRIM family, which has E3 ligase activity and has been demonstrated to be involved in tumor development. In this work, we found that TRIM47 is highly expressed in head and neck squamous cell carcinomas (HNSCC) tissues. TRIM47 overexpression promoted HNSCC cell proliferation. Downregulation of TRIM47 suppressed HNSCC cell proliferation and promoted apoptosis and autophagy. TRIM47 suppression caused cell proliferation inhibition and apoptosis promotion could be reversed by 3-MA, an autophagy inhibitor. In mechanism, TRIM47 interacted with XIAP-associated factor 1 (XAF1), promoting its ubiquitination and degradation. XAF1 promoted HNSCC cell apoptosis and autophagy. TRIM47 overexpression caused cell proliferation promotion and autophagy inhibition could be reversed by XAF1 overexpression. Animal experiments confirmed that the knockdown of TRIM47 inhibits tumor growth in vivo. Ultimately, TRIM47 promotes the ubiquitination and degradation of XAF1 and suppresses apoptosis and autophagy to promote the progression of HNSCC. |
| format | Article |
| id | doaj-art-0e958432b3f046a5a6a2d6adfc7b41fb |
| institution | DOAJ |
| issn | 2589-0042 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj-art-0e958432b3f046a5a6a2d6adfc7b41fb2025-08-20T02:39:48ZengElsevieriScience2589-00422025-01-0128111159010.1016/j.isci.2024.111590TRIM47 promotes head and neck squamous cell carcinoma malignant progression by degrading XAF1 through ubiquitinationChangyun Yu0Chen Zhang1Qianqian Zhang2Cai Zhang3Jingjie Han4Jinying Li5Department of Otolaryngology Head and Neck Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China; Corresponding authorDepartment of Otolaryngology Head and Neck Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, ChinaDepartment of Otolaryngology Head and Neck Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, ChinaDepartment of Otolaryngology Head and Neck Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, ChinaDepartment of Otolaryngology Head and Neck Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, ChinaDepartment of Otolaryngology Head and Neck Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China; Department of Otolaryngology Head and Neck Surgery, Children’s Hospital Affiliated to Zhengzhou University, Zhengzhou 450018, ChinaSummary: Tripartite motif-containing 47 (TRIM47) is a member of the TRIM family, which has E3 ligase activity and has been demonstrated to be involved in tumor development. In this work, we found that TRIM47 is highly expressed in head and neck squamous cell carcinomas (HNSCC) tissues. TRIM47 overexpression promoted HNSCC cell proliferation. Downregulation of TRIM47 suppressed HNSCC cell proliferation and promoted apoptosis and autophagy. TRIM47 suppression caused cell proliferation inhibition and apoptosis promotion could be reversed by 3-MA, an autophagy inhibitor. In mechanism, TRIM47 interacted with XIAP-associated factor 1 (XAF1), promoting its ubiquitination and degradation. XAF1 promoted HNSCC cell apoptosis and autophagy. TRIM47 overexpression caused cell proliferation promotion and autophagy inhibition could be reversed by XAF1 overexpression. Animal experiments confirmed that the knockdown of TRIM47 inhibits tumor growth in vivo. Ultimately, TRIM47 promotes the ubiquitination and degradation of XAF1 and suppresses apoptosis and autophagy to promote the progression of HNSCC.http://www.sciencedirect.com/science/article/pii/S2589004224028177Molecular biologyCell biologyCancer |
| spellingShingle | Changyun Yu Chen Zhang Qianqian Zhang Cai Zhang Jingjie Han Jinying Li TRIM47 promotes head and neck squamous cell carcinoma malignant progression by degrading XAF1 through ubiquitination iScience Molecular biology Cell biology Cancer |
| title | TRIM47 promotes head and neck squamous cell carcinoma malignant progression by degrading XAF1 through ubiquitination |
| title_full | TRIM47 promotes head and neck squamous cell carcinoma malignant progression by degrading XAF1 through ubiquitination |
| title_fullStr | TRIM47 promotes head and neck squamous cell carcinoma malignant progression by degrading XAF1 through ubiquitination |
| title_full_unstemmed | TRIM47 promotes head and neck squamous cell carcinoma malignant progression by degrading XAF1 through ubiquitination |
| title_short | TRIM47 promotes head and neck squamous cell carcinoma malignant progression by degrading XAF1 through ubiquitination |
| title_sort | trim47 promotes head and neck squamous cell carcinoma malignant progression by degrading xaf1 through ubiquitination |
| topic | Molecular biology Cell biology Cancer |
| url | http://www.sciencedirect.com/science/article/pii/S2589004224028177 |
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