An In vitro investigation of Lactobacillus plantarum F4 Strain’s Anti-sickling and Fetal Hemoglobin Inducing Effects on Sickled Erythrocytes
Sickle cell disease (SCD) is a blood illness occurs due to point mutation in the β-globin gene and leads to serious health problems. The aim of the study was to assess probiotic Lactobacillus plantarum F4’s (L. plantarum F4) capacity to induce fetal hemoglobin (HbF) synthesis and anti-sickling effec...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Journal of Pure and Applied Microbiology
2025-03-01
|
| Series: | Journal of Pure and Applied Microbiology |
| Subjects: | |
| Online Access: | https://microbiologyjournal.org/an-in-vitro-investigation-of-lactobacillus-plantarum-f4-strains-anti-sickling-and-fetal-hemoglobin-inducing-effects-on-sickled-erythrocytes/ |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850096061437181952 |
|---|---|
| author | Firdosh Shah Saloni R. Shah Aasruti S. Pramanik Prasant Kumar Naresh C. Laddha Mitesh Dwivedi |
| author_facet | Firdosh Shah Saloni R. Shah Aasruti S. Pramanik Prasant Kumar Naresh C. Laddha Mitesh Dwivedi |
| author_sort | Firdosh Shah |
| collection | DOAJ |
| description | Sickle cell disease (SCD) is a blood illness occurs due to point mutation in the β-globin gene and leads to serious health problems. The aim of the study was to assess probiotic Lactobacillus plantarum F4’s (L. plantarum F4) capacity to induce fetal hemoglobin (HbF) synthesis and anti-sickling effects in sickle erythrocytes isolated from individuals with sickle cell disease. Erythrocytes were isolated from 15 SCD patients and 5 controls blood samples. Erythrocytes were treated with L. plantarum F4 culture supernatant (CS) at concentrations of 30% and 60% (v/v) for up to 120 minutes, following lysozyme treatment to purify the supernatant. The Emmel test was conducted by microscopic observation after treating the samples with different doses and durations of L. plantarum F4 (CS). HPLC was utilized to assess the HbF levels in patients, while normal saline and hydroxyurea served as negative and positive controls. Statistical analyses, including ANOVA and Student’s t-test, were conducted to compare treatment effects. Results demonstrated that at a concentration of 30% (v/v) for 120 minutes, L. plantarum F4 (CS) significantly decreased sickling of SCD erythrocytes (p < 0.0001) and was equivalent to the common medication hydroxyurea. Additionally, compared to the negative control, L. plantarum F4 (CS) considerably raised the HbF% in the erythrocytes of SCD patients (p < 0.0001). For the first time, our study suggests that L. plantarum F4 significantly enhance HbF and anti-sickling activity in sickled erythrocytes, in vitro and can be further explored for in vivo animal model study and developing therapeutics for SCD. |
| format | Article |
| id | doaj-art-0e2f6fcd08a246a894b1d4261341ba8b |
| institution | DOAJ |
| issn | 0973-7510 2581-690X |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Journal of Pure and Applied Microbiology |
| record_format | Article |
| series | Journal of Pure and Applied Microbiology |
| spelling | doaj-art-0e2f6fcd08a246a894b1d4261341ba8b2025-08-20T02:41:18ZengJournal of Pure and Applied MicrobiologyJournal of Pure and Applied Microbiology0973-75102581-690X2025-03-01191307322https://doi.org/10.22207/JPAM.19.1.22An In vitro investigation of Lactobacillus plantarum F4 Strain’s Anti-sickling and Fetal Hemoglobin Inducing Effects on Sickled ErythrocytesFirdosh ShahSaloni R. ShahAasruti S. PramanikPrasant KumarNaresh C. LaddhaMitesh Dwivedihttps://orcid.org/0000-0001-8497-0765Sickle cell disease (SCD) is a blood illness occurs due to point mutation in the β-globin gene and leads to serious health problems. The aim of the study was to assess probiotic Lactobacillus plantarum F4’s (L. plantarum F4) capacity to induce fetal hemoglobin (HbF) synthesis and anti-sickling effects in sickle erythrocytes isolated from individuals with sickle cell disease. Erythrocytes were isolated from 15 SCD patients and 5 controls blood samples. Erythrocytes were treated with L. plantarum F4 culture supernatant (CS) at concentrations of 30% and 60% (v/v) for up to 120 minutes, following lysozyme treatment to purify the supernatant. The Emmel test was conducted by microscopic observation after treating the samples with different doses and durations of L. plantarum F4 (CS). HPLC was utilized to assess the HbF levels in patients, while normal saline and hydroxyurea served as negative and positive controls. Statistical analyses, including ANOVA and Student’s t-test, were conducted to compare treatment effects. Results demonstrated that at a concentration of 30% (v/v) for 120 minutes, L. plantarum F4 (CS) significantly decreased sickling of SCD erythrocytes (p < 0.0001) and was equivalent to the common medication hydroxyurea. Additionally, compared to the negative control, L. plantarum F4 (CS) considerably raised the HbF% in the erythrocytes of SCD patients (p < 0.0001). For the first time, our study suggests that L. plantarum F4 significantly enhance HbF and anti-sickling activity in sickled erythrocytes, in vitro and can be further explored for in vivo animal model study and developing therapeutics for SCD.https://microbiologyjournal.org/an-in-vitro-investigation-of-lactobacillus-plantarum-f4-strains-anti-sickling-and-fetal-hemoglobin-inducing-effects-on-sickled-erythrocytes/sickle cell disease (scd)sickled erythrocytesl. plantarumanti-sickling activityfetal hemoglobin (hbf)hydroxyurea |
| spellingShingle | Firdosh Shah Saloni R. Shah Aasruti S. Pramanik Prasant Kumar Naresh C. Laddha Mitesh Dwivedi An In vitro investigation of Lactobacillus plantarum F4 Strain’s Anti-sickling and Fetal Hemoglobin Inducing Effects on Sickled Erythrocytes Journal of Pure and Applied Microbiology sickle cell disease (scd) sickled erythrocytes l. plantarum anti-sickling activity fetal hemoglobin (hbf) hydroxyurea |
| title | An In vitro investigation of Lactobacillus plantarum F4 Strain’s Anti-sickling and Fetal Hemoglobin Inducing Effects on Sickled Erythrocytes |
| title_full | An In vitro investigation of Lactobacillus plantarum F4 Strain’s Anti-sickling and Fetal Hemoglobin Inducing Effects on Sickled Erythrocytes |
| title_fullStr | An In vitro investigation of Lactobacillus plantarum F4 Strain’s Anti-sickling and Fetal Hemoglobin Inducing Effects on Sickled Erythrocytes |
| title_full_unstemmed | An In vitro investigation of Lactobacillus plantarum F4 Strain’s Anti-sickling and Fetal Hemoglobin Inducing Effects on Sickled Erythrocytes |
| title_short | An In vitro investigation of Lactobacillus plantarum F4 Strain’s Anti-sickling and Fetal Hemoglobin Inducing Effects on Sickled Erythrocytes |
| title_sort | in vitro investigation of lactobacillus plantarum f4 strain s anti sickling and fetal hemoglobin inducing effects on sickled erythrocytes |
| topic | sickle cell disease (scd) sickled erythrocytes l. plantarum anti-sickling activity fetal hemoglobin (hbf) hydroxyurea |
| url | https://microbiologyjournal.org/an-in-vitro-investigation-of-lactobacillus-plantarum-f4-strains-anti-sickling-and-fetal-hemoglobin-inducing-effects-on-sickled-erythrocytes/ |
| work_keys_str_mv | AT firdoshshah aninvitroinvestigationoflactobacillusplantarumf4strainsantisicklingandfetalhemoglobininducingeffectsonsicklederythrocytes AT salonirshah aninvitroinvestigationoflactobacillusplantarumf4strainsantisicklingandfetalhemoglobininducingeffectsonsicklederythrocytes AT aasrutispramanik aninvitroinvestigationoflactobacillusplantarumf4strainsantisicklingandfetalhemoglobininducingeffectsonsicklederythrocytes AT prasantkumar aninvitroinvestigationoflactobacillusplantarumf4strainsantisicklingandfetalhemoglobininducingeffectsonsicklederythrocytes AT nareshcladdha aninvitroinvestigationoflactobacillusplantarumf4strainsantisicklingandfetalhemoglobininducingeffectsonsicklederythrocytes AT miteshdwivedi aninvitroinvestigationoflactobacillusplantarumf4strainsantisicklingandfetalhemoglobininducingeffectsonsicklederythrocytes AT firdoshshah invitroinvestigationoflactobacillusplantarumf4strainsantisicklingandfetalhemoglobininducingeffectsonsicklederythrocytes AT salonirshah invitroinvestigationoflactobacillusplantarumf4strainsantisicklingandfetalhemoglobininducingeffectsonsicklederythrocytes AT aasrutispramanik invitroinvestigationoflactobacillusplantarumf4strainsantisicklingandfetalhemoglobininducingeffectsonsicklederythrocytes AT prasantkumar invitroinvestigationoflactobacillusplantarumf4strainsantisicklingandfetalhemoglobininducingeffectsonsicklederythrocytes AT nareshcladdha invitroinvestigationoflactobacillusplantarumf4strainsantisicklingandfetalhemoglobininducingeffectsonsicklederythrocytes AT miteshdwivedi invitroinvestigationoflactobacillusplantarumf4strainsantisicklingandfetalhemoglobininducingeffectsonsicklederythrocytes |