Investigation of Algerian <i>Crataegus monogyna Jacq</i> Phenolic Compounds (Using LC-ESI-MS/MS Analysis, Antioxidant Activity, and Enzyme Inhibition) and Their Potential Implications for Food and Nutraceutical Applications
Investigations into the phenolic constituents of the butanolic fraction of <i>Crataegus monogyna</i> were optimized using LC-ESI-MS/MS analysis, identifying and quantifying at least 23 fingerprint phytochemical compounds. The major phenolic compounds were epicatechin (99.916 ± 2.208 mg/g...
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2024-11-01
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| author | Sabrina Goudjil Samira Boussekine Sarra Goudjil Hanane Goudjil Mustafa Abdullah Yilmaz Mohammad Shamsul Ola Ahmad Ali Oguz Cakir |
| author_facet | Sabrina Goudjil Samira Boussekine Sarra Goudjil Hanane Goudjil Mustafa Abdullah Yilmaz Mohammad Shamsul Ola Ahmad Ali Oguz Cakir |
| author_sort | Sabrina Goudjil |
| collection | DOAJ |
| description | Investigations into the phenolic constituents of the butanolic fraction of <i>Crataegus monogyna</i> were optimized using LC-ESI-MS/MS analysis, identifying and quantifying at least 23 fingerprint phytochemical compounds. The major phenolic compounds were epicatechin (99.916 ± 2.208 mg/g), isoquercetrin (53.31 ± 1.172 mg/g), chlorogenic acid (47.457 ± 1.010 mg/g), quinic acid (37.819 ± 1.406 mg/g), rutin (29.98 ± 0.740 mg/g), hesperidin (5.296 ± 0.177 mg/g, detected for the first time in the <i>C. monogyna</i> species), astragalin (1.774 ± 0.020 mg/g), and nicotiflorin (1.482 ± 0.016 mg/g). The antioxidant properties of the lyophilized butanolic fraction were evaluated using DPPH, GOR, ABTS, CUPRAC, and reducing power assays, all of which demonstrated that there was strong activity. Additionally, the neuroprotective effect was evaluated in vitro, showing a potent inhibitory effect on acetylcholinesterase (AChE) with an IC50 of 43.65 ± 2.10 µg/mL. The antidiabetic effect was investigated through <i>α</i>-amylase inhibition (IC50 = 91.19 ± 0.10 µg/mL), showing high inhibitory activity. In addition, the butanolic extract exhibited significant urease inhibition with an IC50 of 26.36 ± 0.05 µg/mL. These results suggest that Algerian <i>C. monogyna</i> has potential as a therapeutic agent for managing diabetes complications and as a natural source of AChE inhibitors, making it a promising subject for the treatment of urease-related conditions. Its high concentrations of natural antioxidants, such as epicatechin, isoquercetrin, chlorogenic acid, quinic acid, rutin, hesperidin, and astragalin, make it suitable for integration into medicine, pharmaceuticals, cosmetics, and the food sector. |
| format | Article |
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| institution | OA Journals |
| issn | 2076-3921 |
| language | English |
| publishDate | 2024-11-01 |
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| spelling | doaj-art-0deb009f787d49c8909b49ddff8047de2025-08-20T01:53:52ZengMDPI AGAntioxidants2076-39212024-11-011311135010.3390/antiox13111350Investigation of Algerian <i>Crataegus monogyna Jacq</i> Phenolic Compounds (Using LC-ESI-MS/MS Analysis, Antioxidant Activity, and Enzyme Inhibition) and Their Potential Implications for Food and Nutraceutical ApplicationsSabrina Goudjil0Samira Boussekine1Sarra Goudjil2Hanane Goudjil3Mustafa Abdullah Yilmaz4Mohammad Shamsul Ola5Ahmad Ali6Oguz Cakir7Laboratory of Bioactive Molecules and Applications, Department of Applied Biology, Faculty of Exact Sciences and Natural and Life Sciences, Echahid Cheikh Larbi Tebessi University, Tebessa 12000, AlgeriaLaboratory of Bioactive Molecules and Applications, Department of Applied Biology, Faculty of Exact Sciences and Natural and Life Sciences, Echahid Cheikh Larbi Tebessi University, Tebessa 12000, AlgeriaLaboratory of Didactics of Physical and Chemical Sciences and Applications, Assia Djebar Higher Normal School of Constantine, Constantine 25000, AlgeriaDepartment of Mining Engineering, Mining Operations, Faculty of Engineering, Karadeniz Technical University, Trabzon 61080, TurkiyeDepartment of Analytical Chemistry, Faculty of Pharmacy, Dicle University, Diyarbakir 21280,TurkiyeDepartment of Biochemistry, Faculty of Science, King Saud University, Riyadh 11415, Saudi ArabiaDepartment of Life Sciences, University of Mumbai, Vidyanagari, Mumbai 400098, IndiaDepartment of Analytical Chemistry, Faculty of Pharmacy, Dicle University, Diyarbakir 21280,TurkiyeInvestigations into the phenolic constituents of the butanolic fraction of <i>Crataegus monogyna</i> were optimized using LC-ESI-MS/MS analysis, identifying and quantifying at least 23 fingerprint phytochemical compounds. The major phenolic compounds were epicatechin (99.916 ± 2.208 mg/g), isoquercetrin (53.31 ± 1.172 mg/g), chlorogenic acid (47.457 ± 1.010 mg/g), quinic acid (37.819 ± 1.406 mg/g), rutin (29.98 ± 0.740 mg/g), hesperidin (5.296 ± 0.177 mg/g, detected for the first time in the <i>C. monogyna</i> species), astragalin (1.774 ± 0.020 mg/g), and nicotiflorin (1.482 ± 0.016 mg/g). The antioxidant properties of the lyophilized butanolic fraction were evaluated using DPPH, GOR, ABTS, CUPRAC, and reducing power assays, all of which demonstrated that there was strong activity. Additionally, the neuroprotective effect was evaluated in vitro, showing a potent inhibitory effect on acetylcholinesterase (AChE) with an IC50 of 43.65 ± 2.10 µg/mL. The antidiabetic effect was investigated through <i>α</i>-amylase inhibition (IC50 = 91.19 ± 0.10 µg/mL), showing high inhibitory activity. In addition, the butanolic extract exhibited significant urease inhibition with an IC50 of 26.36 ± 0.05 µg/mL. These results suggest that Algerian <i>C. monogyna</i> has potential as a therapeutic agent for managing diabetes complications and as a natural source of AChE inhibitors, making it a promising subject for the treatment of urease-related conditions. Its high concentrations of natural antioxidants, such as epicatechin, isoquercetrin, chlorogenic acid, quinic acid, rutin, hesperidin, and astragalin, make it suitable for integration into medicine, pharmaceuticals, cosmetics, and the food sector.https://www.mdpi.com/2076-3921/13/11/1350<i>Crataegus monogyna Jacq</i>LC-ESI-MS/MSbutanolic fractionpolyphenolhesperidinurease |
| spellingShingle | Sabrina Goudjil Samira Boussekine Sarra Goudjil Hanane Goudjil Mustafa Abdullah Yilmaz Mohammad Shamsul Ola Ahmad Ali Oguz Cakir Investigation of Algerian <i>Crataegus monogyna Jacq</i> Phenolic Compounds (Using LC-ESI-MS/MS Analysis, Antioxidant Activity, and Enzyme Inhibition) and Their Potential Implications for Food and Nutraceutical Applications Antioxidants <i>Crataegus monogyna Jacq</i> LC-ESI-MS/MS butanolic fraction polyphenol hesperidin urease |
| title | Investigation of Algerian <i>Crataegus monogyna Jacq</i> Phenolic Compounds (Using LC-ESI-MS/MS Analysis, Antioxidant Activity, and Enzyme Inhibition) and Their Potential Implications for Food and Nutraceutical Applications |
| title_full | Investigation of Algerian <i>Crataegus monogyna Jacq</i> Phenolic Compounds (Using LC-ESI-MS/MS Analysis, Antioxidant Activity, and Enzyme Inhibition) and Their Potential Implications for Food and Nutraceutical Applications |
| title_fullStr | Investigation of Algerian <i>Crataegus monogyna Jacq</i> Phenolic Compounds (Using LC-ESI-MS/MS Analysis, Antioxidant Activity, and Enzyme Inhibition) and Their Potential Implications for Food and Nutraceutical Applications |
| title_full_unstemmed | Investigation of Algerian <i>Crataegus monogyna Jacq</i> Phenolic Compounds (Using LC-ESI-MS/MS Analysis, Antioxidant Activity, and Enzyme Inhibition) and Their Potential Implications for Food and Nutraceutical Applications |
| title_short | Investigation of Algerian <i>Crataegus monogyna Jacq</i> Phenolic Compounds (Using LC-ESI-MS/MS Analysis, Antioxidant Activity, and Enzyme Inhibition) and Their Potential Implications for Food and Nutraceutical Applications |
| title_sort | investigation of algerian i crataegus monogyna jacq i phenolic compounds using lc esi ms ms analysis antioxidant activity and enzyme inhibition and their potential implications for food and nutraceutical applications |
| topic | <i>Crataegus monogyna Jacq</i> LC-ESI-MS/MS butanolic fraction polyphenol hesperidin urease |
| url | https://www.mdpi.com/2076-3921/13/11/1350 |
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