EFFECT OF SPERM CAPACITATION MEDIA ON IN VITRO FERTILIZATION OF ARABI EWES OOCYTES
The study was conducted at Assisted Reproductive Technology Center (A R T) College of Medicine, University of Basra , from January , 2009 to January ,2010 . The aim was to study the effect of capacitation media on in vitro fertilization of Arabi ewes oocytes . A total of 2279 oocytes were collec...
Saved in:
| Main Author: | |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
College of Agriculture
2012-03-01
|
| Series: | Mesopotamia Journal of Agriculture |
| Online Access: | https://magrj.mosuljournals.com/article_63474_1709f39537d85f89a10dc34e1e8d261d.pdf |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | The study was conducted at Assisted Reproductive Technology Center (A R T) College of Medicine, University of Basra , from January , 2009 to January ,2010 . The aim was to study the effect of capacitation media on in vitro fertilization of Arabi ewes oocytes . A total of 2279 oocytes were collected from ovaries obtained from Basra Slaughter House .Oocytes were matured in TCM199 (tissue culture medium 199) supplemented with10%Fetal bovin serum (FBS) to study the effect of capacitation media on their fertilizing ability. freshly ejaculated and frozen thawed spermatozoa were capacitated in 1 of 2 media .A Bracket culture medium or Earle’s balanced salt medium . Results revealed that Bracket medium with Hyalouronidase enzyme and Gentamycin instead of Penicillin antibiotics showed significant increase (P≤ 0.05) in number of in vitro fertilized and divided ewe’s oocytes as compared with the Earle’s in both fresh and frozen thawed spermatozoa . |
|---|---|
| ISSN: | 1815-316X 2224-9796 |