PEG-PLGA nanoparticles deposited in Pseudomonas aeruginosa and Burkholderia cenocepacia
In our prior research, polymer nanoparticles (NPs) containing tobramycin displayed robust antibacterial efficacy against biofilm-embedded Pseudomonas aeruginosa (P. aeruginosa) and Burkholderia cenocepacia (B. cenocepacia) cells, critical pathogens in cystic fibrosis. In the current study, we invest...
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Elsevier
2024-12-01
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| Series: | Journal of Pharmaceutical Analysis |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095177924000273 |
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| author | Tinatini Tchatchiashvili Helena Duering Lisa Mueller-Boetticher Christian Grune Dagmar Fischer Mathias W. Pletz Oliwia Makarewicz |
| author_facet | Tinatini Tchatchiashvili Helena Duering Lisa Mueller-Boetticher Christian Grune Dagmar Fischer Mathias W. Pletz Oliwia Makarewicz |
| author_sort | Tinatini Tchatchiashvili |
| collection | DOAJ |
| description | In our prior research, polymer nanoparticles (NPs) containing tobramycin displayed robust antibacterial efficacy against biofilm-embedded Pseudomonas aeruginosa (P. aeruginosa) and Burkholderia cenocepacia (B. cenocepacia) cells, critical pathogens in cystic fibrosis. In the current study, we investigated the deposition of a nanoparticulate carrier composed of poly(d,l-lactic-co-glycolic acid) (PLGA) and poly(ethylene glycol)-block-PLGA (PEG-PLGA) that was either covalently bonded with cyanine-5-amine (Cy5) or noncovalently bound with freely embedded cationic rhodamine B (RhB), which served as a drug surrogate. After exposing these NPs to bacteria, we performed cell fractionation and fluorescence analysis, which highlighted the accumulation of Cy5 in the outer membranes (OMs) and the accumulation of RhB in the cytoplasm (CP) of cells. The results indicated that these organic NPs are effective vehicles for targeted antibiotic delivery in bacterial cells, explaining the observed increase in the efficacy of encapsulated tobramycin against biofilms. This work emphasizes the potential of PEG-PLGA-based formulations for advanced drug delivery strategies. |
| format | Article |
| id | doaj-art-0cee9b339a2d4b53af3b71b7a79c938b |
| institution | Kabale University |
| issn | 2095-1779 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Pharmaceutical Analysis |
| spelling | doaj-art-0cee9b339a2d4b53af3b71b7a79c938b2025-01-30T05:13:57ZengElsevierJournal of Pharmaceutical Analysis2095-17792024-12-011412100939PEG-PLGA nanoparticles deposited in Pseudomonas aeruginosa and Burkholderia cenocepaciaTinatini Tchatchiashvili0Helena Duering1Lisa Mueller-Boetticher2Christian Grune3Dagmar Fischer4Mathias W. Pletz5Oliwia Makarewicz6Institute of Infectious Disease and Infection Control, Jena University Hospital, Jena, 07747, Germany; Corresponding author.Institute of Infectious Disease and Infection Control, Jena University Hospital, Jena, 07747, GermanyInstitute of Pharmaceutical Technology and Biopharmacy, Friedrich-Schiller-University Jena, Jena, 07743, GermanyInstitute of Pharmaceutical Technology and Biopharmacy, Friedrich-Schiller-University Jena, Jena, 07743, GermanyDivision of Pharmaceutical Technology and Biopharmacy, Department of Chemistry and Pharmacy, Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen, 91058, Germany; FAU NeW – Research Center for New Bioactive Compounds, Erlangen, 91058, GermanyInstitute of Infectious Disease and Infection Control, Jena University Hospital, Jena, 07747, GermanyInstitute of Infectious Disease and Infection Control, Jena University Hospital, Jena, 07747, GermanyIn our prior research, polymer nanoparticles (NPs) containing tobramycin displayed robust antibacterial efficacy against biofilm-embedded Pseudomonas aeruginosa (P. aeruginosa) and Burkholderia cenocepacia (B. cenocepacia) cells, critical pathogens in cystic fibrosis. In the current study, we investigated the deposition of a nanoparticulate carrier composed of poly(d,l-lactic-co-glycolic acid) (PLGA) and poly(ethylene glycol)-block-PLGA (PEG-PLGA) that was either covalently bonded with cyanine-5-amine (Cy5) or noncovalently bound with freely embedded cationic rhodamine B (RhB), which served as a drug surrogate. After exposing these NPs to bacteria, we performed cell fractionation and fluorescence analysis, which highlighted the accumulation of Cy5 in the outer membranes (OMs) and the accumulation of RhB in the cytoplasm (CP) of cells. The results indicated that these organic NPs are effective vehicles for targeted antibiotic delivery in bacterial cells, explaining the observed increase in the efficacy of encapsulated tobramycin against biofilms. This work emphasizes the potential of PEG-PLGA-based formulations for advanced drug delivery strategies.http://www.sciencedirect.com/science/article/pii/S2095177924000273PolyesterTobramycinPoly(ethylene glycol)Antimicrobial activityCystic fibrosisBacterial membrane |
| spellingShingle | Tinatini Tchatchiashvili Helena Duering Lisa Mueller-Boetticher Christian Grune Dagmar Fischer Mathias W. Pletz Oliwia Makarewicz PEG-PLGA nanoparticles deposited in Pseudomonas aeruginosa and Burkholderia cenocepacia Journal of Pharmaceutical Analysis Polyester Tobramycin Poly(ethylene glycol) Antimicrobial activity Cystic fibrosis Bacterial membrane |
| title | PEG-PLGA nanoparticles deposited in Pseudomonas aeruginosa and Burkholderia cenocepacia |
| title_full | PEG-PLGA nanoparticles deposited in Pseudomonas aeruginosa and Burkholderia cenocepacia |
| title_fullStr | PEG-PLGA nanoparticles deposited in Pseudomonas aeruginosa and Burkholderia cenocepacia |
| title_full_unstemmed | PEG-PLGA nanoparticles deposited in Pseudomonas aeruginosa and Burkholderia cenocepacia |
| title_short | PEG-PLGA nanoparticles deposited in Pseudomonas aeruginosa and Burkholderia cenocepacia |
| title_sort | peg plga nanoparticles deposited in pseudomonas aeruginosa and burkholderia cenocepacia |
| topic | Polyester Tobramycin Poly(ethylene glycol) Antimicrobial activity Cystic fibrosis Bacterial membrane |
| url | http://www.sciencedirect.com/science/article/pii/S2095177924000273 |
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