Lactococcus lactis NCC 2287 Alleviates Food Allergic Manifestations in Sensitized Mice by Reducing IL-13 Expression Specifically in the Ileum
Objective. Utilizing a food allergy murine model, we have investigated the intrinsic antiallergic potential of the Lactococcus lactis NCC 2287 strain. Methods. BALB/c mice were sensitized at weekly intervals with ovalbumin (OVA) plus cholera toxin (CT) by the oral route for 7 weeks. In this model,...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | Clinical and Developmental Immunology |
| Online Access: | http://dx.doi.org/10.1155/2012/485750 |
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| author | Adrian W. Zuercher Marietta Weiss Sébastien Holvoet Mireille Moser Hélène Moussu Laurence van Overtvelt Stéphane Horiot Philippe Moingeon Sophie Nutten Guénolée Prioult Anurag Singh Annick Mercenier |
| author_facet | Adrian W. Zuercher Marietta Weiss Sébastien Holvoet Mireille Moser Hélène Moussu Laurence van Overtvelt Stéphane Horiot Philippe Moingeon Sophie Nutten Guénolée Prioult Anurag Singh Annick Mercenier |
| author_sort | Adrian W. Zuercher |
| collection | DOAJ |
| description | Objective. Utilizing a food allergy murine model, we have investigated the intrinsic antiallergic potential of the Lactococcus lactis NCC 2287 strain.
Methods. BALB/c mice were sensitized at weekly intervals with ovalbumin (OVA) plus cholera toxin (CT) by the oral route for 7 weeks. In this model, an oral challenge with a high dose of OVA at the end of the sensitization period leads to clinical symptoms. Lactococcus lactis NCC 2287 was given to mice via the drinking water during sensitization (prevention phase) or after sensitization (management phase). Results. Lactococcus lactis NCC 2287 administration to sensitized mice strikingly reduced allergic manifestations in the management phase upon challenge, when compared to control mice. No preventive effect was observed with the strain. Lactococcus lactis NCC 2287 significantly decreased relative expression levels of the Th-2 cytokine, IL-13, and associated chemokines CCL11 (eotaxin-1) and CCL17 (TARC) in the ileum. No effect was observed in the jejunum. Conclusion/Significance. These results taken together designate Lactococcus lactis NCC 2287 as a candidate probiotic strain appropriate in the management of allergic symptoms. |
| format | Article |
| id | doaj-art-0ca38a748220444da1a8d0d97ba5e8b5 |
| institution | Kabale University |
| issn | 1740-2522 1740-2530 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Clinical and Developmental Immunology |
| spelling | doaj-art-0ca38a748220444da1a8d0d97ba5e8b52025-02-03T05:50:08ZengWileyClinical and Developmental Immunology1740-25221740-25302012-01-01201210.1155/2012/485750485750Lactococcus lactis NCC 2287 Alleviates Food Allergic Manifestations in Sensitized Mice by Reducing IL-13 Expression Specifically in the IleumAdrian W. Zuercher0Marietta Weiss1Sébastien Holvoet2Mireille Moser3Hélène Moussu4Laurence van Overtvelt5Stéphane Horiot6Philippe Moingeon7Sophie Nutten8Guénolée Prioult9Anurag Singh10Annick Mercenier11Allergy Group, Department of Nutrition and Health, Nestle Research Center, Vers-chez-les-Blanc, 1000 Lausanne 26, SwitzerlandAllergy Group, Department of Nutrition and Health, Nestle Research Center, Vers-chez-les-Blanc, 1000 Lausanne 26, SwitzerlandAllergy Group, Department of Nutrition and Health, Nestle Research Center, Vers-chez-les-Blanc, 1000 Lausanne 26, SwitzerlandApplied Mathematics Group, Department of Bio-Analytical Sciences, Nestle Research Center, Vers-chez-les-Blanc, 1000 Lausanne 26, SwitzerlandStallergènes SA, Research and Development, 6 rue Alexis de Tocqueville, 92183 Antony, FranceStallergènes SA, Research and Development, 6 rue Alexis de Tocqueville, 92183 Antony, FranceStallergènes SA, Research and Development, 6 rue Alexis de Tocqueville, 92183 Antony, FranceStallergènes SA, Research and Development, 6 rue Alexis de Tocqueville, 92183 Antony, FranceAllergy Group, Department of Nutrition and Health, Nestle Research Center, Vers-chez-les-Blanc, 1000 Lausanne 26, SwitzerlandAllergy Group, Department of Nutrition and Health, Nestle Research Center, Vers-chez-les-Blanc, 1000 Lausanne 26, SwitzerlandAllergy Group, Department of Nutrition and Health, Nestle Research Center, Vers-chez-les-Blanc, 1000 Lausanne 26, SwitzerlandAllergy Group, Department of Nutrition and Health, Nestle Research Center, Vers-chez-les-Blanc, 1000 Lausanne 26, SwitzerlandObjective. Utilizing a food allergy murine model, we have investigated the intrinsic antiallergic potential of the Lactococcus lactis NCC 2287 strain. Methods. BALB/c mice were sensitized at weekly intervals with ovalbumin (OVA) plus cholera toxin (CT) by the oral route for 7 weeks. In this model, an oral challenge with a high dose of OVA at the end of the sensitization period leads to clinical symptoms. Lactococcus lactis NCC 2287 was given to mice via the drinking water during sensitization (prevention phase) or after sensitization (management phase). Results. Lactococcus lactis NCC 2287 administration to sensitized mice strikingly reduced allergic manifestations in the management phase upon challenge, when compared to control mice. No preventive effect was observed with the strain. Lactococcus lactis NCC 2287 significantly decreased relative expression levels of the Th-2 cytokine, IL-13, and associated chemokines CCL11 (eotaxin-1) and CCL17 (TARC) in the ileum. No effect was observed in the jejunum. Conclusion/Significance. These results taken together designate Lactococcus lactis NCC 2287 as a candidate probiotic strain appropriate in the management of allergic symptoms.http://dx.doi.org/10.1155/2012/485750 |
| spellingShingle | Adrian W. Zuercher Marietta Weiss Sébastien Holvoet Mireille Moser Hélène Moussu Laurence van Overtvelt Stéphane Horiot Philippe Moingeon Sophie Nutten Guénolée Prioult Anurag Singh Annick Mercenier Lactococcus lactis NCC 2287 Alleviates Food Allergic Manifestations in Sensitized Mice by Reducing IL-13 Expression Specifically in the Ileum Clinical and Developmental Immunology |
| title | Lactococcus lactis NCC 2287 Alleviates Food Allergic Manifestations in Sensitized Mice by Reducing IL-13 Expression Specifically in the Ileum |
| title_full | Lactococcus lactis NCC 2287 Alleviates Food Allergic Manifestations in Sensitized Mice by Reducing IL-13 Expression Specifically in the Ileum |
| title_fullStr | Lactococcus lactis NCC 2287 Alleviates Food Allergic Manifestations in Sensitized Mice by Reducing IL-13 Expression Specifically in the Ileum |
| title_full_unstemmed | Lactococcus lactis NCC 2287 Alleviates Food Allergic Manifestations in Sensitized Mice by Reducing IL-13 Expression Specifically in the Ileum |
| title_short | Lactococcus lactis NCC 2287 Alleviates Food Allergic Manifestations in Sensitized Mice by Reducing IL-13 Expression Specifically in the Ileum |
| title_sort | lactococcus lactis ncc 2287 alleviates food allergic manifestations in sensitized mice by reducing il 13 expression specifically in the ileum |
| url | http://dx.doi.org/10.1155/2012/485750 |
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